PROTOCOL Dry Ice Self-Pressurized Rapid Freezing (DryIce SPRF): all you need to cool for cryofixation is dry ice: PDF & PPT show
PROTOCOL High pressure freezing and Freeze Substitution of Nematodes: High Pressure Freezing: EMPact (Leica) followed by AFS: Automatic Freeze Substitution (Leica)
PROTOCOL Self-Pressurised Rapid Freezing (SPRF): an easy-to-use and low-cost alternative cryo-fixation method for nematodes
