Hidden diversity in mushrooms explored: A new nematode species, Neodiplogaster unguispiculata sp n. (Rhabditida, Diplogastridae), with a key to the species of Neodiplogaster

Neodiplogaster unguispiculata n. sp. is based on morphological and molecular data. N. unguispiculata sp. n. was isolated from Chondrostereum purpureum in Lappersheide, Tessenderlo, Belgium and cultured on potato dextrose agar seeded with fungi. N. unguispiculata sp. n. is characterised by a unique combination of the following traits: the morphology of the spicules that are distally claw-like bending ventrally, the shape of the gubernaculum that envelope the spicules, the well-developed peloderan bursa with a notched tip, a bursa spanning from anterior the cloaca to tail tip, and by the long, filiform female tail. N. unguispiculata sp. n. shows stomatal dimorphism. Phylogenetic analyses shows that monophyly of Neodiplogaster is maximally supported by both maximum likelihood and Bayesian interference methods. Various species delimitation methods appear to produce a variety of different species hypotheses in Neodiplogaster. Our study shows that molecular can support different species hypotheses. Finally, a key to the species of Neodiplogaster is provided. (C) 2018 Elsevier GmbH. All rights reserved.