Comparison of immunogold staining after self-pressurised rapid freezing, high pressure freezing and classical chemical fixation. This is an example of our method to measure the density of immonlabeling, see this page
For the fixation of SPRF and HPF, see Claeys et al. 2017. For the chemical fixation:
2,5% Glutaraldehyde in 0,05M sodium cacodylate buffer (overnight), postfixation 1% Osmium tetroxide for 1h, dehydrated in ethanol series, en bloc with 1% Uranyl acetate for 1 hour, embedded in Spurr
