NEMATODES

taxonomy, morphology and phylogeny

Revision of HPF, SPRF and DryIce SPRF cryo-fixation TEM protocols from Mon, 2021-01-18 10:16

PROTOCOL High pressure freezing and Freeze Substitution of Nematodes:  High Pressure Freezing: EMPact (Leica) followed by AFS: Automatic Freeze Substitution (Leica)

PROTOCOL Self-Pressurised Rapid Freezing (SPRF):  an easy-to-use and low-cost alternative cryo-fixation method for nematodes

PROTOCOL Dry Ice Self-Pressurized Rapid Freezing (DryIce SPRF):  all you need to cool for cryofixation is dry ice

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith