NEMATODES

taxonomy, morphology and phylogeny

SCANNING ELECTRON-MICROSCOPY OF THE OUTER AND INNER SURFACE OF THE BUCCAL CAVITY OF SOME MONONCHIDA

Publication Type:Journal Article
Year of Publication:1995
Authors:G. Borgonie, Diressche, R., Coomans, A.
Journal:Fundamental and Applied NematologyFundamental and Applied Nematology
Volume:18
Pagination:1-10
ISBN Number:1164-5571
Accession Number:WOS:A1995QK14300001
Abstract:

The buccal cavity of six Mononchida was studied using a combination of dissection and extraction to view the inner and outer structures of the buccal cavity with respect to the surrounding tissue. The extraction of the buccal cavities was done by using a solution consisting of beta-mercapto-ethanol and sodium dodecyl sulphate. As these chemicals denature proteins, they allowed elimination of all surrounding tissue of the buccal cavity, making possible detailed study of its surface using Scanning Electron Microscopy. This solution offers the advantage, over the use of the hypochlorite solution, that prolonged incubation does not lead to a loss of fine detail. The extraction of the buccal cavity showed that the outer surface does contain considerable fine detail and some more massive structures previously unreported. The buccal cavity can be divided into two areas: an anterior area with parallel sides and oblique basal area. This subdivision is arbitrary but it distinguishes between the more or less straight dorsal and two ventrosublateral vertical buccal plates that may carry teeth and the bottom of the buccal cavity, formed by three oblique basal plates, curved towards the centre. The presence of completely detached stoma plates (one dorsal and two ventrosublateral plates) and completely detached basal plates from stoma plates in Anatonchus tridentatus distinguishes it from the other Mononchids studied. A possible functional explanation is discussed.

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