| Publication Type: | Journal Article |
| Year of Publication: | 2000 |
| Authors: | B. Vancoppenolle, Claeys, M., Borgonie, G., Tytgat, T., Coomans, A. |
| Journal: | Microscopy Research and TechniqueMicroscopy Research and Technique |
| Volume: | 49 |
| Pagination: | 212-216 |
| Date Published: | Apr |
| ISBN Number: | 1059-910X |
| Accession Number: | WOS:000086906800015 |
| Abstract: | Various fixation methods for transmission electron microscopy (TEM) were tested on Caenorhabditis elegans embryos. By combining various techniques, using 3.4% chitinase in combination with 1% alpha-chymotripsin, we were able to establish a new fixation procedure that for the first time preserves both membranes and internal cellular ultrastructure of C. elegans embryos in different stages of development. This unique procedure will enable a hitherto unattainable standard for TEM research of C. elegans embryos. Sectioning of specific developmental stages fixed with this method allows a detailed study of ultrastructural aspects of embryogenesis. Microsc. Res. Tech. 49:212-216, 2000. (C) 2000 Wiley-Liss, Inc. |