NEMATODES

taxonomy, morphology and phylogeny

A new preparation method to study fresh plant structures with X-ray computed tomography

Publication Type:Journal Article
Year of Publication:2009
Authors:O. Leroux, Leroux, F., Bellefroid, E., Claeys, M., Couvreur, M., Borgonie, G., Van Hoorebeke, L., Masschaele, B., Viane, R.
Journal:Journal of Microscopy-OxfordJournal of Microscopy-Oxford
Volume:233
Pagination:1-4
Date Published:Jan
ISBN Number:0022-2720
Accession Number:WOS:000262511900001
Abstract:

Since the development of X-ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good-quality images. Therefore, we present a new preparation method for fresh material which includes critical point drying and heavy metal staining. This technique enhances the contrast of fresh tissues, prevents artefacts such as tissue compression, and requires no embedding.

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith