TY - JOUR T1 - An early record of Meloidogyne fallax from Ireland JF - ZookeysZookeys Y1 - 2017 A1 - Topalović, O. A1 - Moore, J. F. A1 - Janssen, T. A1 - Bert, W. A1 - Karssen, G. SP - 33 EP - 52 AB - Root-knot nematodes, Meloidogyne spp., cause huge economic losses worldwide. Currently, three Meloidogyne spp. are present on the quarantine A2 list of EPPO, M. chitwoodi, M. fallax and M. enterolobii. As a quarantine organism, M. fallax has been detected in England and Northern Ireland on sport turf in 2011, and in England on leek in 2013. However, its presence in Ireland has probably been overlooked since 1965, when Mr. John F. Moore and Dr. Mary T. Franklin had detected a new Meloidogyne species for that time. While the relevant data was recorded and a preliminary manuscript describing the species was prepared but never submitted for publication, and together with the original slides, pictures and drawings, it was restudied recently. We compared the population of Irish Meloidogyne sp. to other similar Meloidogyne spp. Careful observation and comparison shows that it belongs to M. fallax. The characters found to be common for Irish Meloidogyne sp. and M. fallax are female stylet length (14.6 mu m) with oval to rounded basal knobs, oval shaped perineal pattern with moderately high dorsal arch, slender stylet in males (18.5 mu m) with set off and rounded basal knobs, slightly set off male head with one post-labial annule and incomplete transverse incisures, and second-stage juveniles with large and rounded stylet basal knobs, and a gradually tapering tail (46.9 mu m) with a broadly rounded tip and a clearly delimitated smooth hyaline part sometimes marked by constrictions (12.9 mu m). The host test and gall formation also correspond to M. fallax. The identification could not be additionally supported by molecular analysis, as we were unable to extract DNA from the old permanent slides. Nevertheless, our study reveals that the Meloidogyne species detected in Ireland in 1965 belongs to M. fallax. VL - 643 SN - 1313-2989 N1 - Topalovic, Olivera Moore, John F. Janssen, Toon Bert, Wim Karssen, Gerrit ER - TY - JOUR T1 - Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis JF - PloS ONEPloS ONE Y1 - 2017 A1 - Janssen, T. A1 - Karssen, G. A1 - Topalović, O. A1 - Coyne, D. A1 - Bert, W. VL - http://dx.doi.org/10.1371/journal.pone.0172190 ER - TY - JOUR T1 - Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution JF - Scientific ReportsScientific Reports Y1 - 2016 A1 - Janssen, T. A1 - Karssen, G. A1 - Verhaeven, M. A1 - Coyne, D. A1 - Bert, W. VL - doi: 10.1038/srep22591 ER - TY - JOUR T1 - First Report of Meloidogyne enterolobii causing tuber galling damage on white yam (Dioscorea rotundata) in Nigeria JF - Plant DiseasePlant Disease Y1 - 2016 A1 - Kolombia, Y. A. A1 - Lava Kumar, P. A1 - Claudius-Cole, A. O. A1 - Karssen, G. A1 - Viaene, N. A1 - Coyne, D. A1 - Bert, W. ER - TY - JOUR T1 - Deceptive morphological variation in Hirschmanniella mucronata (Nematoda: Pratylenchidae) and a polytomous key to the genus JF - NematologyNematology Y1 - 2015 A1 - Khun, K. A1 - Decraemer, W. A1 - Couvreur, M. A1 - Karssen, G. A1 - Steel, H. A1 - Bert, W. SP - 377 EP - 400 ER - TY - JOUR T1 - The cellular structure of the female reproductive system within the Heteroderinae and Meloidogyninae (Nematoda) JF - NematologyNematology Y1 - 2002 A1 - Bert, W. A1 - Karssen, G. A1 - Van Driessche, R. A1 - Geraert, E. SP - 953 EP - 963 KW - Afenestrata KW - CELLS KW - CLASSIFICATION KW - CYST KW - DNA KW - Evolution KW - female genital system KW - GENITAL SYSTEM KW - Globodera KW - GLOBODERA-ROSTOCHIENSIS KW - gonad KW - HETERODERA KW - HETERODERINAE KW - Meloidodera KW - MELOIDOGYNE KW - morphology KW - Nematoda KW - REPRODUCTIVE-SYSTEM KW - SEM KW - SPERMATHECA KW - TYLENCHIDA KW - variable cell number AB - Gonads from living young females, representing 23 different species, were extracted to study the cellular structure of the female genital structure within the Meloidogyninae and Heteroderinae. All genera studied can be characterised by their cellular spermatheca morphology. Within Meloidogyne a spherical spermatheca is found with lobe-like protruding cells, most species having 16 to 18 spermatheca cells with interlaced cell boundaries while M. microtyla and M. ichinohei have more spermatheca cells with different cell boundaries. Heterodera and Globodera reveal a comparable gonad structure. The spermatheca cells of Heterodera are columnar and arranged in a restricted number of rows, whereas in Globodera the spermatheca cells are squarish to rounded, depending on the species. The gonad morphology of Afenestrata koreana is clearly different from what would be expected based on the related genera Globodera and Heterodera. The apparently simplest genital system was found in Meloidodera floridensis where the uterus has a limited number of cells. In the other genera studied a large and variable cell-number was found VL - 4 SN - 1388-5545 N1 - Times Cited: 4 Article English Bert, W State Univ Ghent, Dept Biol, Ledeganckstr 35, B-9000 Ghent, Belgium Cited References Count: 40 651JE BRILL ACADEMIC PUBLISHERS PLANTIJNSTRAAT 2, P O BOX 9000, 2300 PA LEIDEN, NETHERLANDS LEIDEN Part 8 ER - TY - CHAP T1 - Phylogeny and evolution of nematodes T2 - COST Action 872 Exploiting genomics to understand plant-nematode interactions Y1 - 2011 A1 - Bert, W. A1 - Karssen, G. A1 - Helder, J. ED - Jones, J. ED - Gheysen, G. ED - Fenoll, C. SP - 45 EP - 59 JF - COST Action 872 Exploiting genomics to understand plant-nematode interactions PB - Springer CY - Dordrecht VL - 557 ER - TY - JOUR T1 - Diversity and incidence of plant-parasitic nematodes in Belgian turf grass JF - NematologyNematology Y1 - 2011 A1 - Vandenbossche, B. A1 - Viaene, N. A1 - de Sutter, N. A1 - Maes, M. A1 - Karssen, G. A1 - Bert, W. SP - 245 EP - 256 AB - Eleven golf courses and eight football pitches, located in Belgium, were surveyed for plant-parasitic nematodes. This revealed a remarkably high diversity: 52 different species/taxa were identified morphologically, belonging to 23 genera and nine families. Among the most prevalent nematodes on both sports field types were Helicotylenchus pseudorobustus and Meloidogyne naasi (> 85% and > 45% of the analysed samples, respectively). Golf greens contained a higher density and diversity of cyst nematodes than football fields. Cyst nematode species included Punctodera punctata, Heterodera mani, H. ustinovi, H. bifenestra and H. hordecalis. Multivariate statistics of the first three species showed that they could be separated based on morphometrical characters of juveniles, but not on values based on the vulva! pattern. Several detected species, e.g., Meloidogyne minor, are a potential source of infestation of agricultural fields. This study shows the importance of plant-parasitic nematodes in turf grass in temperate Europe. In addition, a comparison between two extraction methods revealed that significantly more nematodes were extracted with the zonal centrifuge than after 2 weeks extraction with the Baermann method, except for juveniles of Meloidogyne spp. and cyst-forming species. VL - 13 SN - 1388-5545 N1 - Vandenbossche, Bart Viaene, Nicole de Sutter, Nancy Maes, Martine Karssen, Gerrit Bert, WimPart 2 ER - TY - JOUR T1 - Deceptive morphological variation in Hirschmanniella mucronata (Nematoda: Pratylenchidae) and a polytomous key to the genus JF - Nematology, in pressNematology, in press Y1 - 2015 A1 - Khun, K. A1 - Decraemer. W. A1 - Couvreur, M. A1 - Karssen, G. A1 - Steel, H. A1 - Bert, W. ER -