01636nas a2200181   4500008004100000020001400041245011400055210006900169300001200238490000700250520106700257100001701324700001501341700002001356700001601376700001601392856004601408       1995                        eng d  a1164-557100aPRESENCE OF PERITROPHIC-LIKE MEMBRANES IN THE INTESTINE OF 3 BACTERIOPHAGOUS NEMATODES (NEMATODA, RHABDITIDA)0 aPRESENCE OF PERITROPHICLIKE MEMBRANES IN THE INTESTINE OF 3 BACT  a227-2330 v183 aUltrastructural analysis shows the presence of membranes originating at the top of the intestinal microvilli, along the entire length of the intestine in the three rhabditid nematodes Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus. The membranes allow the passage of fluorescein isothiocyanate, methyl red, neutral red and acridine orange, but allow only sparse passage of ferritin molecules. Upon the introduction of a sublethal dose of sodium azide, the intestinal lumen displays an increased secretion of membrane layers. Whole mount staining of the nematodes with the Solanum tuberosum and Triticum vulgare lectins, known to bind with high affinity to chitin, shows only specific binding of Solanum tuberosum lectin to the brush border in all developmental stages of C. elegans, P. superbus and A. maximus. The results reveal the presence of membranes in the intestine of three species of one of the most ancient metazoan phyla, exhibiting morphological and functional characteristics reminiscent of peritrophic membranes in insects.1 aBorgonie, G.1 aClaeys, M.1 aVanfleteren, J.1 aDeWaele, D.1 aCoomans, A.  uhttps://nematodes.myspecies.info/node/27601738nas a2200169   4500008004100000020001400041245011400055210006900169300001200238490000700250520120100257100001701458700001501475700001601490700001601506856004601522       1995                        eng d  a1164-557100aIN-VIVO AND IN-VITRO CHARACTERIZATION OF THE INTESTINE OF 15 BACTERIOPHAGOUS NEMATODES (NEMATODA, RHABDITIDA)0 aINVIVO AND INVITRO CHARACTERIZATION OF THE INTESTINE OF 15 BACTE  a115-1220 v183 aThe intestine of the fifteen free-living Rhabditida belonging to three different families was studied using three different approaches : i) using three different axenic media, cultivation of all fifteen nematode species was attempted; ii) in vivo analyses were performed by using two vital stains and one fluorescent dye and by comparing staining patterns; iii) in vitro analyses was done using the intestinal markers acid phosphatase, esterase and the lectin of Ricinus communis II. Although the nematodes can be cultured monoxenically on the same bacterium, Escherichia coli, attempts to culture the fifteen nematode species on the same axenic medium failed. Distinct differences are observed between different areas along the intestinal tract by in vivo study using stains. Similar observations were made in vitro using the intestinal marker acid phosphatase and the binding pattern of Ricinus communis II. This was less evident using esterase as a marker, since considerable non-intestinal tissue staining was evident. The data obtained indicate considerable biochemical differences in the intestinal cells between the nematode species, even if the nematode species belong to the same family.1 aBorgonie, G.1 aClaeys, M.1 aDeWaele, D.1 aCoomans, A.  uhttps://nematodes.myspecies.info/node/27701400nas a2200169   4500008004100000020001400041245016400055210006900219300001200288490000700300520081300307100001701120700001501137700001601152700001601168856004601184       1995                        eng d  a1164-557100aULTRASTRUCTURE OF THE INTESTINE OF THE BACTERIOPHAGOUS NEMATODES CAENORHABDITIS-ELEGANS, PANAGROLAIMUS SUPERBUS AND ACROBELOIDES MAXIMUS (NEMATODA, RHABDITIDA)0 aULTRASTRUCTURE OF THE INTESTINE OF THE BACTERIOPHAGOUS NEMATODES  a123-1330 v183 aThe intestine of the three free-living rhabditid nematodes Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus was studied using transmission electron microscopy of sections made at three locations along the intestine. Between the three nematode species, ultrastructural differences in cellular components are minor. However, two cell types present in all three nematode species are positioned differently along the intestinal tract. Furthermore, two different types of secretions into the intestinal lumen were identified, one only at the anterior intestine, the second present along the entire length of the intestinal lumen. F-actin staining of fifteen whole-mount nematodes revealed the presence of a well developed, cross-shaped intestinal muscle, only present in Cephalobid species.1 aBorgonie, G.1 aClaeys, M.1 aDeWaele, D.1 aCoomans, A.  uhttps://nematodes.myspecies.info/node/278