@article {Claeys2017,
	title = {Self-Pressurised Rapid Freezing (SPRF): an easy-to-use and low-cost alternative cryo-fixation method for nematodes},
	journal = {Nematology},
	volume = {19},
	number = {8},
	year = {2017},
	pages = {871{\textendash}881},
	keywords = {acrobeles complexus, Caenorhabditis elegans, for over 50 years, freeze substitution, has been a crucial, high-pressure freezing, immunogold labelling, major, method for studying tissues, msp, sperm protein, spermatozoa, TEM, transmission electron microscopy},
	isbn = {1568541100003},
	issn = {1388-5545},
	doi = {10.1163/15685411-00003093},
	url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003093},
	author = {Claeys, Myriam and Yushin, Vladimir V. and Leunissen, Jan L.M. and Claeys, Jef and Bert, Wim}
}
@article {Claeys2019a,
	title = {Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes},
	journal = {Nematology},
	volume = {22},
	year = {2019},
	pages = {1{\textendash}8},
	abstract = {Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation, and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii . A~comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore, SPRF fixation is proposed as an affordable, relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes.},
	keywords = {a, a hurdle that needs, at the same time, cuticle, eggshell, globodera rostochiensis, heterodera schachtii, in their early life, nematodes are protected by, one would like to, robust eggshell, second-stage juvenile, stages, the eggshell constitutes, to be overcome if, transmission electron microscopy},
	isbn = {1568541100003},
	issn = {1388-5545},
	doi = {10.1163/15685411-00003287},
	author = {Claeys, Myriam and Handayani, Nurul Dwi and Yushin, Vladimir V. and Lestari, Prabowo and Dikin, Antarjo and Helder, Johannes and Bert, Wim}
}
