@article {292,
	title = {TISSUE TREATMENT FOR WHOLE-MOUNT INTERNAL LECTIN STAINING IN THE NEMATODES CAENORHABDITIS-ELEGANS, PANAGROLAIMUS-SUPERBUS AND ACROBELOIDES-MAXIMUS},
	journal = {HistochemistryHistochemistry},
	volume = {101},
	number = {5},
	year = {1994},
	note = {Borgonie, g vandriessche, e link, cd dewaele, d coomans, a},
	month = {Jun},
	pages = {379-384},
	abstract = {Four different fixation schemes, using ten fluorescent-labelled lectins, were investigated for whole mount internal staining of three rhabditid nematodes: Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus. Acetone-only fixation was found to give strong and reproducible staining, which could be prevented either by periodate treatment of the organisms or by specific inhibitory sugars of the lectins under investigation. Whereas the use of either phosphate or TRIS buffers had no effect on the staining pattern or the fluorescence intensity, the incubation time as well as the in cubation temperature affected the staining reaction. The best results were obtained upon overnight incubation at 4 degrees C: the lectin staining could be inhibited in all cases, except for the intestinal brush border of C. elegans by the lectin of Lens culinaris.},
	isbn = {0301-5564},
	author = {Borgonie, G. and VanDriessche, E. and Link, C. D. and DeWaele, D. and Coomans, A.}
}
@article {245,
	title = {Internal lectin binding patterns in the nematodes Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus},
	journal = {Fundamental and Applied NematologyFundamental and Applied Nematology},
	volume = {20},
	number = {2},
	year = {1997},
	note = {Borgonie, G VanDriessche, E Link, CD Claeys, M DeWaele, D Coomans, A},
	pages = {173-186},
	abstract = {Using ten different lectins, the binding patterns were studied in three free-living rhabditid nematodes : Caenorhabditis elegans, Panagrolaimus superbus, and Acrobeloides maximus. Although all the nematode tissues showed binding by one or more of the lectins used, considerable differences were noticed between the three nematode species. Yolk, four of the coelomocytes, and the oocytes bind most often with the lectins used. Although the intestinal brush border interacts with a lot of foreign materials, it stained only with few lectins. The lectin binding pattern of the yolk indicated that, at the time of incorporation of the yolk in embryos or shortly thereafter, a processing of the yolk occurs resulting in changes in lectin-binding characteristics of the yolk protein.},
	isbn = {1164-5571},
	author = {Borgonie, G. and VanDriessche, E. and Link, C. D. and Claeys, M. and DeWaele, D. and Coomans, A.}
}
