@article {171, title = {Cuticular structure of three Bunonema species and first description of the male of B. penardi Stefanski, 1914 (Nematoda : Bunonematidae)}, journal = {NematologyNematology}, volume = {6}, year = {2004}, note = {Times Cited: 0 Article English Borgonie, G State Univ Ghent, Dept Biol, Nematol Sect, KL Ledeganckstr 35, B-9000 Ghent, Belgium Cited References Count: 38 889AI BRILL ACADEMIC PUBLISHERS PLANTIJNSTRAAT 2, P O BOX 9000, 2300 PA LEIDEN, NETHERLANDS LEIDEN Part 4}, pages = {473-484}, abstract = {This study reanalyses some morphological and ultrastructural aspects of three Bunonema species. Specimens of B. penardi, B. hessi and B.franzi were studied using light microscopy and SEM. Bunonema franzi was also studied by TEM. The male of B. penardi is described, this being the first record of males in the genus Bunonema. Additional morphometric data on the females of this species are presented. SEM pictures show details of the deviant amphid position within the Bunonematidae. In contrast to earlier reports, our TEM results show that the evagination of the cuticle in ridges and tubercles is due to the thickening of the median zone of the cuticle. Ultrastructural details of the species-specific polygonal mesh network on the right body side are revealed. The rods that constitute this network lie above the epicuticle and are therefore extracuticular}, keywords = {BODY WALL, cuticle, FINE-STRUCTURE, GENUS, light microscopy, microscopy, morphology, Nematoda, PROTEIN, RHABDITIDA, SEM, STOMA, TEM, tubercle, ultrastructure, YOLK}, isbn = {1388-5545}, author = {Van Gansbeke, R. and Bert, W. and Govaerts, A. and Claeys, M. and Van Driessche, R. and Manhout, J. and Borgonie, G.} } @article {172, title = {Cuticular structure of three Bunonema species and first description of the male of B-penardi Stefanski, 1914 (Nematoda : Bunonematidae)}, journal = {NematologyNematology}, volume = {6}, year = {2004}, note = {Van Gansbeke, R Bert, W Govaerts, A Claeys, M Van Driessche, R Manhout, J Borgonie, GPart 4}, pages = {473-484}, abstract = {This study reanalyses some morphological and ultrastructural aspects of three Bunonema species. Specimens of B. penardi, B. hessi and B.franzi were studied using light microscopy and SEM. Bunonema franzi was also studied by TEM. The male of B. penardi is described, this being the first record of males in the genus Bunonema. Additional morphometric data on the females of this species are presented. SEM pictures show details of the deviant amphid position within the Bunonematidae. In contrast to earlier reports, our TEM results show that the evagination of the cuticle in ridges and tubercles is due to the thickening of the median zone of the cuticle. Ultrastructural details of the species-specific polygonal mesh network on the right body side are revealed. The rods that constitute this network lie above the epicuticle and are therefore extracuticular.}, isbn = {1388-5545}, author = {Van Gansbeke, R. and Bert, W. and Govaerts, A. and Claeys, M. and Van Driessche, R. and Manhout, J. and Borgonie, G.} } @article {187, title = {Comparative morpho-anatomical studies of the female gonoduct within the Pratylenchidae (Nematoda : Tylenchina)}, journal = {NematologyNematology}, volume = {5}, year = {2003}, note = {Times Cited: 2 Article English Bert, W State Univ Ghent, Dept Biol, Ledeganckstr 35, B-9000 Ghent, Belgium Cited References Count: 33 712QZ BRILL ACADEMIC PUBLISHERS PLANTIJNSTRAAT 2, P O BOX 9000, 2300 PA LEIDEN, NETHERLANDS LEIDEN Part 2}, pages = {293-306}, abstract = {The cellular morphology of the gonoduct of six Pratylenchus species, three Pratylenchoides species, Radopholus similis, Zygorylenchus guevarai, Hirschmanniella loofi and Nacobbus aberrans was revealed by dissection and light microscopy. Except for Nacobbus aberrans, all studied species show an overall similarity in gonoduct construction, i.e., an ovary often ending with a ring of cells, an oviduct formed from two rows of four cells and a 12-celled spermatheca followed by a tricolumella containing 16-24 cells. Pratylenchoides magnicauda and Z guevarai did not diverge from the other Pratylenchidae in this respect, although their gonoduct differs from that of Amplimerlinius and Meloidogyne, both formerly postulated as related genera. The spermatheca structure observed in N. aberrans has not been reported elsewhere in the Nematoda, although the uterus is similar to that reported within the Heteroderinae and Meloidogyninae and the uterus comprises more than 300 cells, enlarging from a tricolumella to a polycolumella. Transmission electron microscopy of Z guevarai revealed details of the cytoplasmatic contact between epithelial cells and the germ cells; a finger-like ovarian wall cell extension was found penetrating the oocyte. The oviduct lacks a preformed lumen and comprises eight cells with highly plicated cell membranes. The spermatheca is constructed from flattened wall cells and is followed by columnar uterus cells where evidence of eggshell formation was demonstrated}, keywords = {CELLS, electron microscopy, ELECTRON-MICROSCOPY, GENUS, gonad, gonoduct, HETERODERINAE, Hirschmanniella, LESION NEMATODE, light microscopy, MELOIDOGYNE, microscopy, morphology, Nacobbus, Nematoda, PENETRANS NEMATA, Pratylenchoides, Pratylenchus, REPRODUCTIVE-SYSTEM, SPERMATHECA, TEM, ultrastructure, Zygotylenchus}, isbn = {1388-5545}, author = {Bert, W. and Van Gansbeke, R. and Claeys, M. and Geraert, E. and Borgonie, G.} } @article {149, title = {The somatic female gonad of Cephalobidae (Nematoda): cellular architecture and associated function}, journal = {NematologyNematology}, volume = {9}, year = {2007}, note = {Times Cited: 0 Article English Bert, W Univ Ghent, Dept Biol, Nematol Unit, Ledeganckstr 35, B-9000 Ghent, Belgium Cited References Count: 46 197FO BRILL ACADEMIC PUBLISHERS PLANTIJNSTRAAT 2, P O BOX 9000, 2300 PA LEIDEN, NETHERLANDS LEIDEN Part 2}, pages = {285-297}, abstract = {The female reproductive system of the free-living nematode family Cephalobidae is examined by means of differential interference contrast, scanning electron and fluorescent microscopy. The model nematode Caenorhabditis elegans and the predatory nematode Prionchulus punctatus are also included in this study; the former mainly to test our results with the very detailed knowledge of this model organism, the latter to provide a representative of the more distantly related Enoplea. In this comparative approach, the analysed gonad structures are discussed with respect to their functional and phylogenetic significance. The general cellular composition of the cephalobid gonad - namely an oviduct comprising two rows of four cells, a distinctly offset spermatheca consisting of 8-16 cells, and a uterus composed of distinct cell rows - differs from all known Nematoda except that of the plant-parasitic Tylenchomorpha. Despite the striking evolutionary conservation of the cellular architecture of the cephalobid gonad there is a complex subcellular specialisation, namely a significant and functionally relevant variation in myofilament organisation, both among Cephalobidae and between major groups of nematodes. We demonstrate the presence of microfilaments that vary in pattern among species and that may play an important role in egg propulsion. The phenomenon of endotokia matricida, in which eggs do not leave the female body, is found to be associated with a massive rupture of the cytoskeleton in the uterus wall. The complexity of the myofibril structure and the associated potential to propagate oocytes actively cannot be solely explained by differences in phylogenetic history, but is also linked to body diameter. In the larger Acrobeloides maximus, the proximal end of the ovary sheath is adorned with 12 distinct longitudinal bands, antibody binding positively for paramyosin, while in the smaller Cephalobus cubaensis myofilament organisation is at random}, keywords = {Acrobeloides, Caenorhabditis, Caenorhabditis elegans, caenorhabditis-elegans, CALIFORNIA, CELLS, CEPHALOBIDAE, Cephalobus, Chiloplacus, cytoskeleton, Evolution, FAMILY, gonad, gonoduct, HERMAPHRODITE, LINEAGES, microscopy, MODEL, Nematoda, NEMATODE, nematodes, paramyosin, PHYLOGENETIC SIGNIFICANCE, Prionchulus, propulsion, REDESCRIPTION, REPRODUCTIVE-SYSTEM, SPERMATHECA, THORNE, TYLENCHIDA, Zeldia}, isbn = {1388-5545}, author = {Bert, W. and Vangestel, S. and Houthoofd, W. and Van Gansbeke, R. and Borgonie, G.} }