@article {95, title = {Cytochrome c oxidase subunit 1 analysis of Xiphinema diversicaudatum, X. pachtaicum, X. simile and X. vuittenezi (Nematoda, Dorylaimida)}, journal = {European Journal of Plant PathologyEuropean Journal of Plant Pathology}, volume = {127}, number = {4}, year = {2010}, note = {Kumari, Shesh Decraemer, Wilfrida De Luca, Francesca Tiefenbrunner, Wolfgang}, month = {Aug}, pages = {493-499}, abstract = {Genetic analyses using sequences of partial cytochrome c oxidase subunit 1 (cox1) gene of mitochondrial DNA were conducted to determine the extent of genetic variation within and among Xiphinema diversicaudatum, X. pachtaicum, X. simile and X. vuittenezi populations. Pairwise distance among the four species was 22.5 to 31.2\%. Four different sequence variants of cox1 were determined among six populations of X. diversicaudatum and three variants among three populations of X. simile. Nucleotide variation was detected at 18 of 414 bp (1.9 to 2.7\%) in X. diversicaudatum and 4 of 435 bp (0.2 to 0.9\%) in X. simile. All changes were at silent sites. No nucleotide variation was detected within three populations of X. pachtaicum and within three populations of X. vuittenezi.}, isbn = {0929-1873}, author = {Kumari, S. and Decraemer, W. and De Luca, F. and Tiefenbrunner, W.} } @article {96, title = {Molecular characterization of Xiphinema brevicollum (Nematoda: Longidoridae) from the Czech Republic}, journal = {European Journal of Plant PathologyEuropean Journal of Plant Pathology}, volume = {128}, number = {2}, year = {2010}, note = {Kumari, Shesh Decraemer, Wilfrida De Luca, Francesca}, month = {Oct}, pages = {243-250}, abstract = {Populations of Xiphinema brevicollum occurring in the Czech Republic were described morphologically and molecularly. Published species-specific primer set BL18 and BV3 was used to amplify three populations of X. brevicollum from the Czech Republic. These primers were tested against 9 species of Xiphinema and 11 species of Longidorus. Amplification was also observed for X. inaequale, X. italiae and X. lambertii. Three additional markers, mitochondrial cytochrome c oxidase subunit 1, ribosomal D2/D3 expansion segment of the 28S gene and 18S gene, were amplified and sequenced for X. brevicollum, X. inaequale and X. lambertii belonging to X. americanum-group. Comparison of cox1 sequences of X. brevicollum from the Czech Republic with X. taylori from the Slovak Republic (accession number AM086702) suggested that these populations represent the same species.}, isbn = {0929-1873}, author = {Kumari, S. and Decraemer, W. and De Luca, F.} }