@article {Janssen2017, title = {The pitfalls of molecular species identification: a case study within the genus Pratylenchus (Nematoda: Pratylenchidae)}, journal = {Nematology}, volume = {00}, year = {2017}, pages = {1{\textendash}21}, keywords = {acrobeloides cf, barcoding provide a, DIAGNOSTICS, for, identification of organisms and, mislabelled sequences, MOLECULAR PHYLOGENY, molecular taxonomy and dna, nanus, new synonym, powerful tool for the, pratylenchus flakkensis, pratylenchus goodeyi, pratylenchus lentis n, pratylenchus pratensis, syn, topotypes}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003117}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003117}, author = {Janssen, Toon and Karssen, Gerrit and Couvreur, Marjolein and Waeyenberge, Lieven and Bert, Wim} } @book {Kolombia2017, title = {Morphological and molecular characterisation of Scutellonema species from yam (Dioscorea spp.) and a key to the species of~the~genus}, series = {Nematology}, volume = {19}, number = {7}, year = {2017}, pages = {751{\textendash}787}, keywords = {asiedu, clathricaudatum, coi, crop, cultivated for its edible, d2-d3, DIAGNOSTICS, dioscorea spp, ghana, IDENTIFICATION, is an important staple, KEY, nigeria, phylogeny, scutellonema, scutellonema cavenessi, scutellonema paralabiatum, species delimitation, tubers in west africa, west africa, yam}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003084}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003084}, author = {Kolombia, Yao A. and Karssen, Gerrit and Viaene, Nicole and Kumar, P. Lava and Joos, Lisa and Coyne, Danny L. and Bert, Wim} } @article {Claeys2017, title = {Self-Pressurised Rapid Freezing (SPRF): an easy-to-use and low-cost alternative cryo-fixation method for nematodes}, journal = {Nematology}, volume = {19}, number = {8}, year = {2017}, pages = {871{\textendash}881}, keywords = {acrobeles complexus, Caenorhabditis elegans, for over 50 years, freeze substitution, has been a crucial, high-pressure freezing, immunogold labelling, major, method for studying tissues, msp, sperm protein, spermatozoa, TEM, transmission electron microscopy}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003093}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003093}, author = {Claeys, Myriam and Yushin, Vladimir V. and Leunissen, Jan L.M. and Claeys, Jef and Bert, Wim} } @article {Godjo2018, title = {Steinernema kandii n. sp. (Rhabditida: Steinernematidae), a~new~entomopathogenic nematode from northern Benin}, journal = {Nematology}, year = {2018}, pages = {1{\textendash}22}, keywords = {1934, bicornutum -group, d2-d3, description, entomopathogenic nematodes, epn, have been, its, molecular, morphology, Morphometrics, new species, of the family, phylogeny, RHABDITIDA, steinernematidae filipjev, taxonomy, xenorhabdus indica}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003201}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003201}, author = {Godjo, Anique and Afouda, Leonard and Baimey, Hugues and Couvreur, Marjolein and Zadji, Lionel and Houssou, Gladys and Bert, Wim and Willems, Anne and Decraemer, Wilfrida} } @article {Slos2018, title = {Description of Myolaimus mycophilus Slos \& Bert sp. n. (Rhabditida: Myolaimidae)}, journal = {Nematology}, volume = {20}, number = {2}, year = {2018}, pages = {175{\textendash}186}, abstract = {Copyright {\textcopyright} 2018 by Koninklijke Brill NV, Leiden, The Netherlands. Myolaimus mycophilus Slos \& Bert sp. n. was isolated from mushrooms from different locations in Belgium and cultured on nutrient agar seeded with Escherichia coli OP50. Myolaimus mycophilus sp. n. is characterised by a unique combination of the following characters: adults with lateral fields not differentiated, buccal cavity triangular, cheilostom anisomorphic, female with a post-vulval uterine sac as long as the corresponding body diam., wrinkled eggshell, male with seven pairs of genital papillae, cloacal aperture covered by a trapezoidal cloacal flap and with a single ventral papilla at its base. The new species and M. goodeyorum Andr{\'a}ssy, 1984 were molecularly characterised by sequencing the 18S and D2-D3 28S expansion segments of rDNA. Phylogenetic relationships were reconstructed based on concatenated analyses and the phylogenetic placement of Myolaimus, an intriguing genus within the Rhabditida, is discussed.}, keywords = {Belgium, fungi, Ganoderma applanatum, Laetiporus sulphureus, molecular, morphology, Morphometrics, Myolaimus goodeyorum, new species, phylogeny, taxonomy, Trametes}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003134}, author = {Slos, Dieter and Couvreur, Marjolein and Bert, Wim} } @article {Qing2018a, title = {A new species of Malenchus (Nematoda: Tylenchomorpha) with an updated phylogeny of the Tylenchidae}, journal = {Nematology}, volume = {20}, number = {9}, year = {2018}, pages = {815{\textendash}836}, abstract = {The family Tylenchidae is one of most abundant and diverse nematode groups found in soil habitats. In this study, a new species named Malenchus cylindricus sp. n. is described based on light and scanning electron microscopy and molecular data. The new species is characterised by elongate-cylindrical vulva-anus body shape and a narrow annulation. We updated the phylogeny of Tylenchidae by including molecular data for the rare genera Miculenchus and Tenunemellus and by improving the morphological characterisation of the genus Lelenchus . Additionally, we compared the effect of alignment methods on the tree topologies and branch support values. The results suggest that the position of subfamily Ecphyadophorinae within Tylenchidae needs to be further revised. Phylogenies inferred from different sequence-based alignments were relatively more similar ( i.e. , tree topology and branch support) to one another than to secondary structure-aided methods. Finally, conflicting branch support values can be reconciled by properly selecting the alignment method.}, keywords = {Belgium, China, description, Ecphyadophora, Ecphyadophorinae, Ecphyadophoroides, Filenchus, Lelenchus leptosoma, Malenchus cylindricus sp. n., Mexico, Miculenchus salvus, morphology, Morphometrics, taxonomy, Tenunemellus sheri}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003177}, author = {Qing, Xue and Pereira, Tiago J. and Slos, Dieter and Couvreur, Marjolein and Bert, Wim} } @article {Cortada2019, title = {Integration of nematology as a training and research discipline in sub-Saharan Africa: progress and prospects}, journal = {Nematology}, year = {2019}, pages = {1{\textendash}21}, abstract = {Within sub-Saharan Africa (SSA), although nematodes are viewed among the most important threats to crop production and food security, the presence of trained nematologists working within this discipline has traditionally been viewed as scarce. The few research studies concerning this subject address this topic from a country or sub-regional perspective and generally portray nematology as {\textquoteleft}insufficient{\textquoteright}. Over the past two decades, a few initiatives have been instrumental in building greater nematology expertise. For the first time a structured survey was undertaken, involving interviews with individuals from SSA that were (or currently are) involved in nematology training programmes, research, national extension services or in African universities. This paper provides evidence of the positive impact of various initiatives and shows an increase in the number of available nematology positions, together with high rates of graduates that return home to occupy qualified positions. Our findings will help researchers, policy makers and donors to identify areas requiring support to increase the promotion of nematology in SSA and to make an impact for end-users.}, keywords = {academic curricula, capacity building, education, eumaine, extension services, imanema, pinc, tertiary institutions}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003291}, author = {Cortada, Laura and Dehennin, Inge and Bert, Wim and Coyne, Danny} } @article {Nguyen2019, title = {Molecular and morphological characterisation of a new root-lesion nematode, Pratylenchus horti n. sp. (Tylenchomorpha: Pratylenchidae), from Ghent University Botanical Garden}, journal = {Nematology}, volume = {21}, number = {7}, year = {2019}, pages = {739{\textendash}752}, abstract = {Root-lesion nematodes, Pratylenchus spp., are one of the most important nematode groups in economic terms. The combination of morphological analyses and molecular analyses based on D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA regions supported the establishment of a new Pratylenchus species, making a total of 103 valid Pratylenchus species. The females of P. horti n. sp. are characterised by the following traits: low labial region with two annuli continuous to the body, en face form belonging to group II sensu Corbett \& Clark (1983) with submedian triangular-shaped segments fused with the oral disc and separated from the lateral segments, lateral field with four incisures at vulval level and lacking areolation, robust stylet 15-17 μ m long with rounded knobs, and subcylindrical tail with smooth tail tip. The males are largely similar to the females but differ from the females by the partially areolated lateral field, slightly ventrally arcuate and weakly cephalated spicules (15-19 μ m), and ventrally curved elongate conical tail with a poorly protruding, crenate bursa. The new species was recovered from soil and root samples from the rhizosphere of Hedychium greenii growing in the Botanical Garden, Ghent University, Belgium.}, keywords = {28S, Belgium, cluster analysis, coi, d2-d3, exotic plant, Ginger lily, Hedychium greenii, its, morphology, Morphometrics, mtDNA, new species, plant-parasitic nematode, rDNA, taxonomy, web-based key}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003249}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Wang2019, title = {Aphelenchoides medicagus n. sp. (Tylenchina: Aphelenchoididae) found in Medicago sativa imported into China from the USA}, journal = {Nematology}, volume = {21}, year = {2019}, pages = {709{\textendash}723}, abstract = {Aphelenchoides medicagus n. sp. isolated in Ningbo Port, P.R. China, from dried leaves and shoots of Medicago sativa imported from the USA, is described. It is characterised by a lateral field with four lines (three bands), stylet 9.0-12.0 μ m long, excretory pore situated at same level as, or slightly posterior, to the nerve ring, vagina sclerotised, female vulval flap absent, post-uterine sac short, spicules 9.2-14.2 μ m long (median curved line), rosethorn-shaped, apex and rostrum rounded, poorly developed, female and male tail conical with terminus bearing star-like processes. The new species belongs to the Group 3 category of Aphelenchoides species sensu Shahina. Phylogenetic analyses based on 18S, ITS and 28S D2-D3 of rDNA and mtCOI sequences confirmed its status as a new species and closely related to A. besseyi and A. fujianensis .}, keywords = {alfalfa, description, lucerne, molecular, morphology, Morphometrics, new species, phylogeny, plant-parasitic nematode, quarantine, SEM, taxonomy}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003247}, author = {Wang, Zhen and Bert, Wim and Gu, Jianfeng and Couvreur, Marjolein and Li, Hongmei} } @article {Claeys2019a, title = {Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes}, journal = {Nematology}, volume = {22}, year = {2019}, pages = {1{\textendash}8}, abstract = {Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation, and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii . A~comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore, SPRF fixation is proposed as an affordable, relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes.}, keywords = {a, a hurdle that needs, at the same time, cuticle, eggshell, globodera rostochiensis, heterodera schachtii, in their early life, nematodes are protected by, one would like to, robust eggshell, second-stage juvenile, stages, the eggshell constitutes, to be overcome if, transmission electron microscopy}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003287}, author = {Claeys, Myriam and Handayani, Nurul Dwi and Yushin, Vladimir V. and Lestari, Prabowo and Dikin, Antarjo and Helder, Johannes and Bert, Wim} } @article {Claeys2019, title = {Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes}, journal = {Nematology}, volume = {22}, year = {2020}, pages = {103{\textendash}110}, isbn = {1568541100003}, doi = {10.1163/15685411-00003287}, author = {Claeys, Myriam and Dwi Handayani, Nurul and Yushin, Vladimir V. and Lestari, Prabowo and Dikin, Antarjo and Helder, Johannes and Bert, Wim} }