@article {152, title = {A female of Xiphinema vuittenezi (Nematoda : Longidoridae) with two vulvae and abundant numbers of males in a soil sample}, journal = {NematologyNematology}, volume = {8}, year = {2006}, note = {Kumari, Shesh Decraemer, WilfridaPart 6}, pages = {943-947}, isbn = {1388-5545}, author = {Kumari, S. and Decraemer, W.} } @article {143, title = {The genus Longidorus (Nematoda : Longidoridae) from Bohemia and South Moravia in the rhizosphere of fruit orchards and vineyards}, journal = {HelminthologiaHelminthologia}, volume = {44}, number = {4}, year = {2007}, note = {Kumari, S. Decraemer, W.}, month = {Dec}, pages = {193-203}, abstract = {The occurrence and geographic distribution of the genus Longidorus was investigated during a nematological survey of Bohemia and South Moravia between 2003 and 2006. Soil samples were collected from 139 different fruit orchards and vineyards. Three Longidorus species (L. elongatus, L. euonymus and L. leptocephalus) were recorded. L. euonymus constitutes a new record for the Czech nematofauna.}, isbn = {0440-6605}, author = {Kumari, S. and Decraemer, W.} } @article {120, title = {Molecular and morphological delineation of Longidorus poessneckensis Altherr, 1974 (Nematoda: Dorylaimida)}, journal = {European Journal of Plant PathologyEuropean Journal of Plant Pathology}, volume = {123}, number = {2}, year = {2009}, note = {Kumari, Shesh Decraemer, Wilfrida Traversa, Donato Liskova, Marta}, month = {Feb}, pages = {125-137}, abstract = {The plant-parasitic nematode Longidorus poessneckensis from the Czech Republic was morphologically and molecularly characterised. Molecular analyses were carried out using mitochondrial DNA (cytochrome c oxidase subunit 1-cox1) and ribosomal DNA (ITS2-second internal transcribed spacer, 18S gene and D2/D3 expansion segments of the 28S gene), which were amplified and sequenced. Phylogenetic relationship of L. poessneckensis with three morphologically closely related species, i.e. L. macrosoma, L. helveticus and L. uroshis, was inferred by using maximum likelihood and maximum parsimony methods, with a female of Xiphinema diversicaudatum and a bivulval female of X. vuittenezi as outgroups. All multiple alignments yielded similar basic trees supporting the uniqueness of L. poessneckensis and the validity of the four Longidorus species identified using morphological characters. Phylogenetic analyses revealed that L. poessneckensis is more closely related to L. macrosoma and L. helveticus than to L. uroshis. High inter-population diversity (19\%) was observed across the cox1 gene between two populations of L. poessneckensis.}, isbn = {0929-1873}, author = {Kumari, S. and Decraemer, W. and Traversa, D. and Liskova, M.} } @article {121, title = {A single bivulval female of Xiphinema diversicaudatum and two bivulval females of Xiphinema vuittenezi (Nematoda: Longidoridae)}, journal = {HelminthologiaHelminthologia}, volume = {46}, number = {4}, year = {2009}, note = {Kumari, S. Decraemer, W.}, month = {Dec}, pages = {250-252}, abstract = {A population of Xiphinema vuittenezi from an apple orchard and a population of X. diversicaudatum from a peach orchard yielded bivulval females. Morphometrics of these females are presented.}, isbn = {0440-6605}, author = {Kumari, S. and Decraemer, W.} } @article {80, title = {First report of Trichodorus primitivus, T. sparsus and T. viruliferus (Nematoda: Trichodoridae) from the Czech Republic}, journal = {HelminthologiaHelminthologia}, volume = {48}, number = {3}, year = {2011}, note = {Kumari, S. Decraemer, W.}, month = {Sep}, pages = {195-199}, abstract = {A survey has been carried out to study the occurrence and distribution of Trichodorus primitivus, T. sparsus and T. viruliferus in the Czech Republic under the rhizosphere of orchards, forests, vineyards and strawberry. Total 208 sites were surveyed and only 29 sites were found positive for these species. All three species are reported in the Czech Republic for the first time.}, isbn = {0440-6605}, author = {Kumari, S. and Decraemer, W.} } @article {95, title = {Cytochrome c oxidase subunit 1 analysis of Xiphinema diversicaudatum, X. pachtaicum, X. simile and X. vuittenezi (Nematoda, Dorylaimida)}, journal = {European Journal of Plant PathologyEuropean Journal of Plant Pathology}, volume = {127}, number = {4}, year = {2010}, note = {Kumari, Shesh Decraemer, Wilfrida De Luca, Francesca Tiefenbrunner, Wolfgang}, month = {Aug}, pages = {493-499}, abstract = {Genetic analyses using sequences of partial cytochrome c oxidase subunit 1 (cox1) gene of mitochondrial DNA were conducted to determine the extent of genetic variation within and among Xiphinema diversicaudatum, X. pachtaicum, X. simile and X. vuittenezi populations. Pairwise distance among the four species was 22.5 to 31.2\%. Four different sequence variants of cox1 were determined among six populations of X. diversicaudatum and three variants among three populations of X. simile. Nucleotide variation was detected at 18 of 414 bp (1.9 to 2.7\%) in X. diversicaudatum and 4 of 435 bp (0.2 to 0.9\%) in X. simile. All changes were at silent sites. No nucleotide variation was detected within three populations of X. pachtaicum and within three populations of X. vuittenezi.}, isbn = {0929-1873}, author = {Kumari, S. and Decraemer, W. and De Luca, F. and Tiefenbrunner, W.} } @article {96, title = {Molecular characterization of Xiphinema brevicollum (Nematoda: Longidoridae) from the Czech Republic}, journal = {European Journal of Plant PathologyEuropean Journal of Plant Pathology}, volume = {128}, number = {2}, year = {2010}, note = {Kumari, Shesh Decraemer, Wilfrida De Luca, Francesca}, month = {Oct}, pages = {243-250}, abstract = {Populations of Xiphinema brevicollum occurring in the Czech Republic were described morphologically and molecularly. Published species-specific primer set BL18 and BV3 was used to amplify three populations of X. brevicollum from the Czech Republic. These primers were tested against 9 species of Xiphinema and 11 species of Longidorus. Amplification was also observed for X. inaequale, X. italiae and X. lambertii. Three additional markers, mitochondrial cytochrome c oxidase subunit 1, ribosomal D2/D3 expansion segment of the 28S gene and 18S gene, were amplified and sequenced for X. brevicollum, X. inaequale and X. lambertii belonging to X. americanum-group. Comparison of cox1 sequences of X. brevicollum from the Czech Republic with X. taylori from the Slovak Republic (accession number AM086702) suggested that these populations represent the same species.}, isbn = {0929-1873}, author = {Kumari, S. and Decraemer, W. and De Luca, F.} }