NEMATODES

taxonomy, morphology and phylogeny

Revision of Comparison of immunogold staining after self-pressurised rapid freezing, high pressure freezing and classical chemical fixation from Wed, 2017-07-12 15:37

Comparison of immunogold staining after self-pressurised rapid freezing, high pressure freezing and classical chemical fixation. This is an example of our method to measure the density of immonlabeling, see this page

For the fixation of SPRF and HPF, see Claeys et al. 2017. For the chemical fixation:

2,5% Glutaraldehyde in 0,05M sodium cacodylate buffer (overnight), postfixation 1% Osmium tetroxide for 1h, dehydrated in ethanol series, en bloc with 1% Uranyl acetate for 1 hour, embedded in Spurr

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith