| Publication Type: | Journal Article |
| Year of Publication: | 2009 |
| Authors: | M. Willems, Boone, M., Couvreur, M., De Mulder, K., Van Ranst, J., Artois, T., Borgonie, G. |
| Journal: | Development Genes and EvolutionDevelopment Genes and Evolution |
| Volume: | 219 |
| Pagination: | 273-279 |
| Date Published: | May |
| ISBN Number: | 0949-944X |
| Accession Number: | WOS:000266372500005 |
| Abstract: | A method for studying whole mount flatworm embryos based on freeze-cracking of the eggs is described. This method allows successful immunohistological and immunocytological studies of whole mount embryos. It does not require the use of sharpened needles or a microinjection system to puncture the eggshell. Moreover, this method is more practical and less time-consuming than classical puncturing and much cheaper than the use of a microinjection system. The advantages of this method are illustrated by results of several immunolocalisation experiments in the macrostomid flatworm Macrostomum lignano. The optimal procedure and crucial steps for this method are discussed. |