TY - JOUR T1 - Self-Pressurised Rapid Freezing (SPRF): an easy-to-use and low-cost alternative cryo-fixation method for nematodes JF - Nematology Y1 - 2017 DO - 10.1163/15685411-00003093 A1 - Claeys, Myriam A1 - Yushin, Vladimir V. A1 - Leunissen, Jan L.M. A1 - Claeys, Jef A1 - Bert, Wim SP - 871–881 KW - acrobeles complexus KW - Caenorhabditis elegans KW - for over 50 years KW - freeze substitution KW - has been a crucial KW - high-pressure freezing KW - immunogold labelling KW - major KW - method for studying tissues KW - msp KW - sperm protein KW - spermatozoa KW - TEM KW - transmission electron microscopy VL - 19 SN - 1568541100003 UR - http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003093 ER - TY - JOUR T1 - Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes JF - Nematology Y1 - 2019 DO - 10.1163/15685411-00003287 A1 - Claeys, Myriam A1 - Handayani, Nurul Dwi A1 - Yushin, Vladimir V. A1 - Lestari, Prabowo A1 - Dikin, Antarjo A1 - Helder, Johannes A1 - Bert, Wim SP - 1–8 KW - a KW - a hurdle that needs KW - at the same time KW - cuticle KW - eggshell KW - globodera rostochiensis KW - heterodera schachtii KW - in their early life KW - nematodes are protected by KW - one would like to KW - robust eggshell KW - second-stage juvenile KW - stages KW - the eggshell constitutes KW - to be overcome if KW - transmission electron microscopy AB - Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation, and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii . A comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore, SPRF fixation is proposed as an affordable, relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes. VL - 22 SN - 1568541100003 ER - TY - JOUR T1 - Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes JF - Nematology Y1 - 2020 DO - 10.1163/15685411-00003287 A1 - Claeys, Myriam A1 - Dwi Handayani, Nurul A1 - Yushin, Vladimir V. A1 - Lestari, Prabowo A1 - Dikin, Antarjo A1 - Helder, Johannes A1 - Bert, Wim SP - 103–110 VL - 22 SN - 1568541100003 ER -