01119nas a2200349 4500008004100000020001800041022001400059245011900073210006900192300001400261490000700275653002400282653002700306653002200333653002400355653002300379653002700402653002500429653001000454653003200464653000800496653001800504653001600522653000800538653003700546100001900583700002500602700002500627700001600652700001400668856008700682 2017 eng d a1568541100003 a1388-554500aSelf-Pressurised Rapid Freezing (SPRF): an easy-to-use and low-cost alternative cryo-fixation method for nematodes0 aSelfPressurised Rapid Freezing SPRF an easytouse and lowcost alt a871–8810 v1910aacrobeles complexus10aCaenorhabditis elegans10afor over 50 years10afreeze substitution10ahas been a crucial10ahigh-pressure freezing10aimmunogold labelling10amajor10amethod for studying tissues10amsp10asperm protein10aspermatozoa10aTEM10atransmission electron microscopy1 aClaeys, Myriam1 aYushin, Vladimir, V.1 aLeunissen, Jan, L.M.1 aClaeys, Jef1 aBert, Wim uhttp://booksandjournals.brillonline.com/content/journals/10.1163/15685411-0000309300865nas a2200349 4500008004100000245001000041210001000051300001100061490000700072653001700079653001500096653001300111653003000124653003100154653000800185653001000193653001800203653001500221653001000236653000800246653001600254653002300270653002600293653002800319653002400347653002400371653003000395653000800425653001900433100001700452856004600469 2017 eng d00aCo Co0 aCo Co a1–140 v0010a1937 are in-10abicornutum10achitwood10aentomopathogenic nematode10aentomopathogenic nematodes10aepn10agonad10ain the family10amorphology10aovary10aSEM10asteinernema10asteinernema affine10asteinernema arenarium10asteinernema carpocapsae10asteinernema feltiae10asteinernema glaseri10asteinernematidae chitwood10aTEM10aultrastructure1 aJemec, Anita uhttps://nematodes.myspecies.info/node/75601940nas a2200397 4500008004100000020001400041245015500055210006900210300001200279490000600291520086500297653001401162653001201176653001901188653001001207653002101217653001501238653001501253653001301268653001201281653001501293653000801308653001001316653000801326653001301334653001901347653000901366100002101375700001301396700001701409700001501426700002201441700001601463700001701479856004601496 2004 eng d a1388-554500aCuticular structure of three Bunonema species and first description of the male of B. penardi Stefanski, 1914 (Nematoda : Bunonematidae)0 aCuticular structure of threei Bunonemai species and first descri a473-4840 v63 aThis study reanalyses some morphological and ultrastructural aspects of three Bunonema species. Specimens of B. penardi, B. hessi and B.franzi were studied using light microscopy and SEM. Bunonema franzi was also studied by TEM. The male of B. penardi is described, this being the first record of males in the genus Bunonema. Additional morphometric data on the females of this species are presented. SEM pictures show details of the deviant amphid position within the Bunonematidae. In contrast to earlier reports, our TEM results show that the evagination of the cuticle in ridges and tubercles is due to the thickening of the median zone of the cuticle. Ultrastructural details of the species-specific polygonal mesh network on the right body side are revealed. The rods that constitute this network lie above the epicuticle and are therefore extracuticular10aBODY WALL10acuticle10aFINE-STRUCTURE10aGENUS10alight microscopy10amicroscopy10amorphology10aNematoda10aPROTEIN10aRHABDITIDA10aSEM10aSTOMA10aTEM10atubercle10aultrastructure10aYOLK1 aVan Gansbeke, R.1 aBert, W.1 aGovaerts, A.1 aClaeys, M.1 aVan Driessche, R.1 aManhout, J.1 aBorgonie, G. uhttps://nematodes.myspecies.info/node/17102725nas a2200457 4500008004100000020001400041245011500055210006900170300001200239490000600251520149700257653001001754653002401764653002401788653001001812653001001822653001301832653001801845653002001863653002001883653002101903653001601924653001501940653001501955653001301970653001301983653002101996653002002017653001702037653002402054653001602078653000802094653001902102653001802121100001302139700002102152700001502173700001602188700001702204856004602221 2003 eng d a1388-554500aComparative morpho-anatomical studies of the female gonoduct within the Pratylenchidae (Nematoda : Tylenchina)0 aComparative morphoanatomical studies of the female gonoduct with a293-3060 v53 aThe cellular morphology of the gonoduct of six Pratylenchus species, three Pratylenchoides species, Radopholus similis, Zygorylenchus guevarai, Hirschmanniella loofi and Nacobbus aberrans was revealed by dissection and light microscopy. Except for Nacobbus aberrans, all studied species show an overall similarity in gonoduct construction, i.e., an ovary often ending with a ring of cells, an oviduct formed from two rows of four cells and a 12-celled spermatheca followed by a tricolumella containing 16-24 cells. Pratylenchoides magnicauda and Z guevarai did not diverge from the other Pratylenchidae in this respect, although their gonoduct differs from that of Amplimerlinius and Meloidogyne, both formerly postulated as related genera. The spermatheca structure observed in N. aberrans has not been reported elsewhere in the Nematoda, although the uterus is similar to that reported within the Heteroderinae and Meloidogyninae and the uterus comprises more than 300 cells, enlarging from a tricolumella to a polycolumella. Transmission electron microscopy of Z guevarai revealed details of the cytoplasmatic contact between epithelial cells and the germ cells; a finger-like ovarian wall cell extension was found penetrating the oocyte. The oviduct lacks a preformed lumen and comprises eight cells with highly plicated cell membranes. The spermatheca is constructed from flattened wall cells and is followed by columnar uterus cells where evidence of eggshell formation was demonstrated10aCELLS10aelectron microscopy10aELECTRON-MICROSCOPY10aGENUS10agonad10agonoduct10aHETERODERINAE10aHirschmanniella10aLESION NEMATODE10alight microscopy10aMELOIDOGYNE10amicroscopy10amorphology10aNacobbus10aNematoda10aPENETRANS NEMATA10aPratylenchoides10aPratylenchus10aREPRODUCTIVE-SYSTEM10aSPERMATHECA10aTEM10aultrastructure10aZygotylenchus1 aBert, W.1 aVan Gansbeke, R.1 aClaeys, M.1 aGeraert, E.1 aBorgonie, G. uhttps://nematodes.myspecies.info/node/18702458nas a2200445 4500008004100000020001400041245010800055210006900163300001200232490000700244520128700251653001301538653001901551653001001570653002401580653002401604653001401628653001901642653001001661653001301671653001801684653001601702653001401718653001501732653001501747653001301762653001301775653002101788653001601809653002401825653001601849653001301865653000801878653001601886653001901902100001301921700001501934700001701949856004601966 2006 eng d a0022-300X00aThe comparative cellular architecture of the female gonoduct among Tylenchoidea (Nematoda : Tylenchina)0 acomparative cellular architecture of the female gonoduct among T a362-3750 v383 aThe cellular architecture of the female gonoduct of 68 nematode populations representing 42 species belonging to Tylenchidae, Belonolaimidae, Hoplolaimidae and Meloinema is shown to have an overall similarity in cellular gonoduct structure. The oviduct consists of two rows of four cells; the spermatheca is comprised of 10 to 20 cells, and the uterus cells, except in the case of Psilenchus, are arranged in four (Tylenchidae) or three (Belonolaimidae, Hoplolaimidae and Meloinema) regular rows. Although the genus Meloinema is classified within Meloidogynidae, its spermatheca is clearly hoplolaimid-like and lacks the spherical shape with lobe-like protruding cells typical of Meloidogyne. Detailed morphology of expelled gonoducts may provide a valuable character set in phylogenctic analysis, and the cellular morphology of the spermatheca appears to be a distinguishing feature at species level, especially in the genera Tylenchus and Geocenamus. Ultrastructural data on the oviduct-spermatheca region of Meloidogvne incognita complement light-microscopic (LM) results. The combination of LM of expelled organs and transmission electron microscopy (TEM) on selected sections is put for-ward as a powerful tool to combine three-dimensional knowledge with ultrastructural detail10aanalysis10aBelonolaimidae10aCELLS10aelectron microscopy10aELECTRON-MICROSCOPY10aEvolution10aGENITAL SYSTEM10aGENUS10agonoduct10aHoplolaimidae10aMELOIDOGYNE10aMeloinema10amicroscopy10amorphology10aNematoda10aNEMATODE10aORDER TYLENCHIDA10aPOPULATIONS10aREPRODUCTIVE-SYSTEM10aSPERMATHECA10ataxonomy10aTEM10aTylenchidae10aultrastructure1 aBert, W.1 aClaeys, M.1 aBorgonie, G. uhttps://nematodes.myspecies.info/node/160