@article {Qing2017b, title = {3D printing in zoological systematics: Integrative taxonomy of Labrys chinensis gen. nov., sp. nov. (Nematoda: Tylenchomorpha)}, journal = {Journal of Zoological Systematics and Evolutionary Research}, number = {July}, year = {2017}, keywords = {3d modeling, NEMATODE, new genus, new species, phylogeny, Tylenchidae}, issn = {09475745}, doi = {10.1111/jzs.12191}, url = {http://doi.wiley.com/10.1111/jzs.12191}, author = {Qing, Xue and Bert, Wim} } @article {Janssen2017, title = {The pitfalls of molecular species identification: a case study within the genus Pratylenchus (Nematoda: Pratylenchidae)}, journal = {Nematology}, volume = {00}, year = {2017}, pages = {1{\textendash}21}, keywords = {acrobeloides cf, barcoding provide a, DIAGNOSTICS, for, identification of organisms and, mislabelled sequences, MOLECULAR PHYLOGENY, molecular taxonomy and dna, nanus, new synonym, powerful tool for the, pratylenchus flakkensis, pratylenchus goodeyi, pratylenchus lentis n, pratylenchus pratensis, syn, topotypes}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003117}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003117}, author = {Janssen, Toon and Karssen, Gerrit and Couvreur, Marjolein and Waeyenberge, Lieven and Bert, Wim} } @article {Qing2017a, title = {Molecular phylogeny of Malenchus and Filenchus (Nematoda: Tylenchidae)}, journal = {Zoologica Scripta}, volume = {46}, number = {5}, year = {2017}, pages = {625{\textendash}636}, issn = {03003256}, doi = {10.1111/zsc.12236}, url = {http://doi.wiley.com/10.1111/zsc.12236}, author = {Qing, Xue and Decraemer, Wilfrida and Claeys, Myriam and Bert, Wim} } @book {Kolombia2017, title = {Morphological and molecular characterisation of Scutellonema species from yam (Dioscorea spp.) and a key to the species of~the~genus}, series = {Nematology}, volume = {19}, number = {7}, year = {2017}, pages = {751{\textendash}787}, keywords = {asiedu, clathricaudatum, coi, crop, cultivated for its edible, d2-d3, DIAGNOSTICS, dioscorea spp, ghana, IDENTIFICATION, is an important staple, KEY, nigeria, phylogeny, scutellonema, scutellonema cavenessi, scutellonema paralabiatum, species delimitation, tubers in west africa, west africa, yam}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003084}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003084}, author = {Kolombia, Yao A. and Karssen, Gerrit and Viaene, Nicole and Kumar, P. Lava and Joos, Lisa and Coyne, Danny L. and Bert, Wim} } @article {Claeys2017, title = {Self-Pressurised Rapid Freezing (SPRF): an easy-to-use and low-cost alternative cryo-fixation method for nematodes}, journal = {Nematology}, volume = {19}, number = {8}, year = {2017}, pages = {871{\textendash}881}, keywords = {acrobeles complexus, Caenorhabditis elegans, for over 50 years, freeze substitution, has been a crucial, high-pressure freezing, immunogold labelling, major, method for studying tissues, msp, sperm protein, spermatozoa, TEM, transmission electron microscopy}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003093}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003093}, author = {Claeys, Myriam and Yushin, Vladimir V. and Leunissen, Jan L.M. and Claeys, Jef and Bert, Wim} } @book {Janssen2017a, title = {Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis}, series = {PLoS ONE}, volume = {12}, number = {3}, year = {2017}, pages = {e0172190}, abstract = {During sampling of several Coffea arabica plantations in Tanzania severe root galling, caused by a root-knot nematode was observed. From pure cultures, morphology and mor-phometrics of juveniles and females matched perfectly with Meloidogyne africana, whereas morphology of the males matched identically with those of Meloidogyne decalineata. Based on their Cox1 sequence, however, the recovered juveniles, females and males were con-firmed to belong to the same species, creating a taxonomic conundrum. Adding further to this puzzle, re-examination of M. oteifae type material showed insufficient morphological evi-dence to maintain its status as a separate species. Consequently, M. decalineata and M. otei-fae are synonymized with M. africana, which is herewith redescribed based on results of light and scanning electron microscopy, ribosomal and mitochondrial DNA sequences, isozyme electrophoresis, along with bionomic and cytogenetic features. Multi-gene phylogenetic analy-sis placed M. africana outside of the three major clades, together with M. coffeicola, M. ichino-hei and M. camelliae. This phylogenetic position was confirmed by several morphological features, including cellular structure of the spermatheca, egg mass position, perineal pattern and head shape. Moreover, M. africana was found to be a polyphagous species, demonstrat-ing that " early-branching " Meloidogyne spp. are not as oligophagous as had previously been assumed. Cytogenetic information indicates M. africana (2n = 21) and M. ardenensis (2n = 51{\textendash}54) to be a triploid mitotic parthenogenetic species, revealing at least four independent ori-gins of mitotic parthenogenesis within the genus Meloidogyne. Furthermore, M. mali (n = 12) was found to reproduce by amphimixis, indicating that amphimictic species with a limited num-ber of chromosomes are widespread in the genus, potentially reflecting the ancestral state of the genus. The wide variation in chromosome numbers and associated changes in reproduc-tion modes indicate that cytogenetic evolution played a crucial role in the speciation of root-knot nematodes and plant-parasitic nematodes in general.}, isbn = {1111111111}, issn = {19326203}, doi = {10.1371/journal.pone.0172190}, author = {Janssen, Toon and Karssen, Gerrit and Topalovi{\'c}, Olivera and Coyne, Danny and Bert, Wim} } @article {Topalovic2017, title = {An early record of Meloidogyne fallax from Ireland}, journal = {ZooKeys}, volume = {2017}, number = {643}, year = {2017}, pages = {33{\textendash}52}, abstract = {Root-knot nematodes, Meloidogyne spp., cause huge economic losses worldwide. Currently, three Meloidogyne spp. are present on the quarantine A2 list of EPPO, M. chitwoodi, M. fallax and M. enterolobii. As a quarantine organism, M. fallax has been detected in England and Northern Ireland on sport turf in 2011, and in England on leek in 2013. However, its presence in Ireland has probably been overlooked since 1965, when Mr. John F. Moore and Dr. Mary T. Franklin had detected a new Meloidogyne species for that time. While the relevant data was recorded and a preliminary manuscript describing the species was prepared but never submitted for publication, and together with the original slides, pictures and drawings, it was restudied recently. We compared the population of Irish Meloidogyne sp. to other similar Meloidogyne spp. Careful observation and comparison shows that it belongs to M. fallax. The characters found to be common for Irish Meloidogyne sp. and M. fallax are female stylet length (14.6 μm) with oval to rounded basal knobs, oval shaped perineal pattern with moderately high dorsal arch, slender stylet in males (18.5 μm) with set off and rounded basal knobs, slightly set off male head with one post-labial annule and incomplete transverse incisures, and second-stage juveniles with large and rounded stylet basal knobs, and a gradually tapering tail (46.9 μm) with a broadly rounded tip and a clearly delimitated smooth hyaline part sometimes marked by constrictions (12.9 μm). The host test and gall formation also correspond to M. fallax. The identification could not be additionally supported by molecular analysis, as we were unable to extract DNA from the old permanent slides. Nevertheless, our study reveals that the Meloidogyne species detected in Ireland in 1965 belongs to M. fallax.}, keywords = {CHARACTERS, Cork, Host, morphology, Morphometrics, Root-knot nematode}, issn = {13132970}, doi = {10.3897/zookeys.643.11266}, author = {Topalovi{\'c}, Olivera and Moore, John F. and Janssen, Toon and Bert, Wim and Karssen, Gerrit} } @article {Slos2017, title = {Caenorhabditis monodelphis sp. n.: defining the stem morphology and genomics of the genus Caenorhabditis}, journal = {BMC Zoology}, volume = {2}, number = {1}, year = {2017}, pages = {4}, publisher = {BMC Zoology}, keywords = {De, description, Evolution, Genome, phylogeny, systematics, taxonomy}, issn = {2056-3132}, doi = {10.1186/s40850-017-0013-2}, url = {http:///bmczool.biomedcentral.com/articles/10.1186/s40850-017-0013-2}, author = {Slos, Dieter and Sudhaus, Walter and Stevens, Lewis and Bert, Wim and Blaxter, Mark} } @article {Qing2017c, title = {Ultrastructural, phylogenetic and rRNA secondary structural analysis of a new nematode (Nematoda: Tylenchomorpha) from mushroom with recovery of intestinal crystals}, journal = {Zoologischer Anzeiger - A Journal of Comparative Zoology}, volume = {269}, year = {2017}, pages = {13{\textendash}25}, publisher = {Elsevier GmbH.}, issn = {00445231}, doi = {10.1016/j.jcz.2017.06.002}, url = {http://linkinghub.elsevier.com/retrieve/pii/S0044523117300517}, author = {Qing, Xue and Slos, Dieter and Claeys, Myriam and Bert, Wim} } @article {Stegen2017, title = {Drivers of salamander extirpation mediated by Batrachochytrium salamandrivorans}, journal = {Nature}, volume = {544}, number = {7650}, year = {2017}, pages = {353{\textendash}356}, publisher = {Nature Publishing Group}, abstract = {The recent arrival of Batrachochytrium salamandrivorans in Europe was followed by rapid expansion of its geographical distribution and host range, confirming the unprecedented threat that this chytrid fungus poses to western Palaearctic amphibians1,2. Mitigating this hazard requires a thorough understanding of the pathogen{\textquoteright}s disease ecology that is driving the extinction process. Here, we monitored infection, disease and host population dynamics in a Belgian fire salamander (Salamandra salamandra) population for two years immediately after the first signs of infection. We show that arrival of this chytrid is associated with rapid population collapse without any sign of recovery, largely due to lack of increased resistance in the surviving salamanders and a demographic shift that prevents compensation for mortality. The pathogen adopts a dual transmission strategy, with environmentally resistant non-motile spores in addition to the motile spores identified in its sister species B. dendrobatidis. The fungus retains its virulence not only in water and soil, but also in anurans and less susceptible urodelan species that function as infection reservoirs. The combined characteristics of the disease ecology suggest that further expansion of this fungus will behave as a {\textquoteleft}perfect storm{\textquoteright} that is able to rapidly extirpate highly susceptible salamander populations across Europe.}, issn = {0028-0836}, doi = {10.1038/nature22059}, url = {http://www.nature.com/doifinder/10.1038/nature22059}, author = {Stegen, Gwij and Pasmans, Frank and Schmidt, Benedikt R. and Rouffaer, Lieze O. and Van Praet, Sarah and Schaub, Michael and Canessa, Stefano and Laudelout, Arnaud and Kinet, Thierry and Adriaensen, Connie and Haesebrouck, Freddy and Bert, Wim and Bossuyt, Franky and Martel, An} } @article {Janssen2017b, title = {Molecular characterization and species delimiting of plant-parasitic nematodes of the genus Pratylenchus from the penetrans group (Nematoda: Pratylenchidae)}, journal = {Molecular Phylogenetics and Evolution}, year = {2017}, abstract = {Root-lesion nematodes of the genus Pratylenchus are an important pest parasitizing a wide range of vascular plants including several economically important crops. However, morphological diagnosis of the more than 100 species is problematic due to the low number of diagnostic features, high morphological plasticity and incomplete taxonomic descriptions. In order to employ barcoding based diagnostics, a link between morphology and species specific sequences has to be established. In this study, we reconstructed a multi-gene phylogeny of the Penetrans group using nuclear ribosomal and mitochondrial gene sequences. A combination of this phylogenetic framework with molecular species delineation analysis, population genetics, morphometric information and sequences from type location material allowed us to establish the species boundaries within the Penetrans group and as such clarify long-standing controversies about the taxonomic status of P. penetrans, P. fallax and P. convallariae. Our study also reveals a remarkable amount of cryptic biodiversity within the genus Pratylenchus confirming that identification on morphology alone can be inconclusive in this taxonomically confusing genus.}, keywords = {Cryptic species complex, DNA Barcoding, phylogeny, Pratylenchus arlingtoni, Root-lesion nematodes, species delimitation}, issn = {10959513}, doi = {10.1016/j.ympev.2017.07.027}, url = {http://dx.doi.org/10.1016/j.ympev.2017.07.027}, author = {Janssen, Toon and Karssen, Gerrit and Orlando, Valeria and Subbotin, Sergei A. and Bert, Wim} } @article {DeSwaef2017, title = {Ultrastructural morphology of the envelope of Dover sole Solea solea eggs from fertilization until hatching with emphasis on sample preparation}, journal = {Micron}, volume = {99}, year = {2017}, pages = {9{\textendash}18}, publisher = {Elsevier Ltd}, issn = {09684328}, doi = {10.1016/j.micron.2017.03.014}, url = {http://linkinghub.elsevier.com/retrieve/pii/S0968432817300380}, author = {De Swaef, Evelien and Claeys, Myriam and Bert, Wim and Huysseune, Ann and Witten, Paul Eckhard and Van den Broeck, Wim and Decostere, Annemie} } @article {Qing2017, title = {Ultrastructural, phylogenetic and rRNA secondary structural analysis of a new nematode (Nematoda: Tylenchomorpha) from mushroom with recovery of intestinal crystals}, journal = {Zoologischer Anzeiger - A Journal of Comparative Zoology}, volume = {269}, year = {2017}, pages = {13{\textendash}25}, publisher = {Elsevier GmbH.}, issn = {00445231}, doi = {10.1016/j.jcz.2017.06.002}, url = {http://linkinghub.elsevier.com/retrieve/pii/S0044523117300517}, author = {Qing, Xue and Slos, Dieter and Claeys, Myriam and Bert, Wim} } @article {Miraeiz2017, title = {Aphelenchoides gorganensis n. sp. (Nematoda: Aphelenchoididae), a new species from Iran}, journal = {European Journal of Plant Pathology}, volume = {149}, number = {1}, year = {2017}, pages = {157{\textendash}169}, publisher = {European Journal of Plant Pathology}, keywords = {LSU rDNA, morphology, Morphometrics, new species, SEM, taxonomy}, issn = {15738469}, doi = {10.1007/s10658-017-1175-z}, author = {Miraeiz, Esmaeil and Heydari, Ramin and Bert, Wim} } @article {Miraeiz2017a, title = {Aphelenchoides gorganensis n. sp. (Nematoda: Aphelenchoididae), a new species from Iran}, journal = {European Journal of Plant Pathology}, volume = {149}, number = {1}, year = {2017}, month = {sep}, pages = {157{\textendash}169}, issn = {0929-1873}, doi = {10.1007/s10658-017-1175-z}, url = {http://link.springer.com/10.1007/s10658-017-1175-z}, author = {Miraeiz, Esmaeil and Heydari, Ramin and Bert, Wim} } @article {VanVaerenbergh2017, title = {Effect of Ion Exchange Resin Functionality on Catalytic Activity and Leaching of Palladium Nanoparticles in Suzuki Cross-Coupling Reactions}, journal = {ChemCatChem}, volume = {9}, number = {3}, year = {2017}, pages = {451{\textendash}457}, keywords = {heterogeneous catalysis, ion exchange resins, palladium nanoparticles, Suzuki cross-coupling reactions}, issn = {18673899}, doi = {10.1002/cctc.201601175}, author = {Van Vaerenbergh, Beau and Lauwaert, Jeroen and Bert, Wim and Thybaut, Joris W. and De Clercq, Jeriffa and Vermeir, Pieter} } @article {Sanchez-Monge2017, title = {mtCOI successfully diagnoses the four main plant-parasitic Aphelenchoides species (Nematoda: Aphelenchoididae) and supports a multiple origin of plant-parasitism in this paraphyletic genus}, journal = {European Journal of Plant Pathology}, year = {2017}, publisher = {European Journal of Plant Pathology}, keywords = {Concatenated analysis, Foliar nematodes, Molecular b}, issn = {0929-1873}, doi = {10.1007/s10658-016-1141-1}, url = {http://link.springer.com/10.1007/s10658-016-1141-1}, author = {Sanchez-Monge, Alcides and Janssen, Toon and Fang, Yiwu and Couvreur, Marjolein and Karssen, Gerrit and Bert, Wim} } @article {Herren2018, title = {Compost as a carrier medium for ento nematodes {\textendash} The influence of compost maturity on their virulence and survival}, journal = {Biological Control}, volume = {125}, year = {2018}, month = {oct}, pages = {29{\textendash}38}, issn = {10499644}, doi = {10.1016/j.biocontrol.2018.06.007}, url = {https://linkinghub.elsevier.com/retrieve/pii/S1049964418300732}, author = {Herren, Gis{\`e}le L. and Binnemans, Isabelle and Joos, Lisa and Viaene, Nicole and Ehlers, Ralf-Udo and Vandecasteele, Bart and Bert, Wim and Steel, Hanne} } @article {Godjo2018, title = {Steinernema kandii n. sp. (Rhabditida: Steinernematidae), a~new~entomopathogenic nematode from northern Benin}, journal = {Nematology}, year = {2018}, pages = {1{\textendash}22}, keywords = {1934, bicornutum -group, d2-d3, description, entomopathogenic nematodes, epn, have been, its, molecular, morphology, Morphometrics, new species, of the family, phylogeny, RHABDITIDA, steinernematidae filipjev, taxonomy, xenorhabdus indica}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003201}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003201}, author = {Godjo, Anique and Afouda, Leonard and Baimey, Hugues and Couvreur, Marjolein and Zadji, Lionel and Houssou, Gladys and Bert, Wim and Willems, Anne and Decraemer, Wilfrida} } @article {ISI:000449120200008, title = {Hidden diversity in mushrooms explored: A new nematode species, Neodiplogaster unguispiculata sp n. (Rhabditida, Diplogastridae), with a key to the species of Neodiplogaster}, journal = {Zoologischer Anzeiger}, volume = {276}, year = {2018}, month = {sep}, pages = {71{\textendash}85}, publisher = {ELSEVIER GMBH, URBAN \& FISCHER VERLAG}, type = {Article}, address = {OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY}, abstract = {Neodiplogaster unguispiculata n. sp. is based on morphological and molecular data. N. unguispiculata sp. n. was isolated from Chondrostereum purpureum in Lappersheide, Tessenderlo, Belgium and cultured on potato dextrose agar seeded with fungi. N. unguispiculata sp. n. is characterised by a unique combination of the following traits: the morphology of the spicules that are distally claw-like bending ventrally, the shape of the gubernaculum that envelope the spicules, the well-developed peloderan bursa with a notched tip, a bursa spanning from anterior the cloaca to tail tip, and by the long, filiform female tail. N. unguispiculata sp. n. shows stomatal dimorphism. Phylogenetic analyses shows that monophyly of Neodiplogaster is maximally supported by both maximum likelihood and Bayesian interference methods. Various species delimitation methods appear to produce a variety of different species hypotheses in Neodiplogaster. Our study shows that molecular can support different species hypotheses. Finally, a key to the species of Neodiplogaster is provided. (C) 2018 Elsevier GmbH. All rights reserved.}, keywords = {Evolution, Neodi, phylogeny, systematics, taxonomy}, issn = {0044-5231}, doi = {10.1016/j.jcz.2018.07.004}, author = {Slos, Dieter and Couvreur, Marjolein and Bert, Wim} } @article {Slos2018, title = {Description of Myolaimus mycophilus Slos \& Bert sp. n. (Rhabditida: Myolaimidae)}, journal = {Nematology}, volume = {20}, number = {2}, year = {2018}, pages = {175{\textendash}186}, abstract = {Copyright {\textcopyright} 2018 by Koninklijke Brill NV, Leiden, The Netherlands. Myolaimus mycophilus Slos \& Bert sp. n. was isolated from mushrooms from different locations in Belgium and cultured on nutrient agar seeded with Escherichia coli OP50. Myolaimus mycophilus sp. n. is characterised by a unique combination of the following characters: adults with lateral fields not differentiated, buccal cavity triangular, cheilostom anisomorphic, female with a post-vulval uterine sac as long as the corresponding body diam., wrinkled eggshell, male with seven pairs of genital papillae, cloacal aperture covered by a trapezoidal cloacal flap and with a single ventral papilla at its base. The new species and M. goodeyorum Andr{\'a}ssy, 1984 were molecularly characterised by sequencing the 18S and D2-D3 28S expansion segments of rDNA. Phylogenetic relationships were reconstructed based on concatenated analyses and the phylogenetic placement of Myolaimus, an intriguing genus within the Rhabditida, is discussed.}, keywords = {Belgium, fungi, Ganoderma applanatum, Laetiporus sulphureus, molecular, morphology, Morphometrics, Myolaimus goodeyorum, new species, phylogeny, taxonomy, Trametes}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003134}, author = {Slos, Dieter and Couvreur, Marjolein and Bert, Wim} } @article {Bletz2018, title = {Disruption of skin microbiota contributes to salamander disease}, journal = {Proceedings of the Royal Society B: Biological Sciences}, volume = {285}, number = {1885}, year = {2018}, month = {aug}, pages = {20180758}, issn = {0962-8452}, doi = {10.1098/rspb.2018.0758}, url = {http://rspb.royalsocietypublishing.org/lookup/doi/10.1098/rspb.2018.0758}, author = {Bletz, Molly C. and Kelly, Moira and Sabino-Pinto, Joana and Bales, Emma and Van Praet, Sarah and Bert, Wim and Boyen, Filip and Vences, Miguel and Steinfartz, Sebastian and Pasmans, Frank and Martel, An} } @article {Qing2018, title = {3D printing in zoological systematics: Integrative taxonomy of Labrys chinensis gen. nov., sp. nov. (Nematoda: Tylenchomorpha)}, journal = {Journal of Zoological Systematics and Evolutionary Research}, volume = {56}, number = {1}, year = {2018}, month = {feb}, pages = {35{\textendash}47}, issn = {09475745}, doi = {10.1111/jzs.12191}, url = {http://doi.wiley.com/10.1111/jzs.12191}, author = {Qing, Xue and Bert, Wim} } @article {Yushin2018, title = {The mature and immature spermatozoa of the free- living marine nematode Daptonema sp . (Nematoda : Monhysterida : Xyalidae )}, journal = {Russian Journal of Nematology}, volume = {26}, number = {2}, year = {2018}, pages = {129{\textendash}144}, keywords = {female gonoduct, fibrous bodies, major sperm protein, membranous organelles, microtubules, monhysterina, postvulval uterine sac, pseudopod, sperm activation, spermatogenesis}, author = {Yushin, Vladimir V and Malakhov, Vladimir V and Claeys, Myriam and Bert, Wim} } @article {Qing2018a, title = {A new species of Malenchus (Nematoda: Tylenchomorpha) with an updated phylogeny of the Tylenchidae}, journal = {Nematology}, volume = {20}, number = {9}, year = {2018}, pages = {815{\textendash}836}, abstract = {The family Tylenchidae is one of most abundant and diverse nematode groups found in soil habitats. In this study, a new species named Malenchus cylindricus sp. n. is described based on light and scanning electron microscopy and molecular data. The new species is characterised by elongate-cylindrical vulva-anus body shape and a narrow annulation. We updated the phylogeny of Tylenchidae by including molecular data for the rare genera Miculenchus and Tenunemellus and by improving the morphological characterisation of the genus Lelenchus . Additionally, we compared the effect of alignment methods on the tree topologies and branch support values. The results suggest that the position of subfamily Ecphyadophorinae within Tylenchidae needs to be further revised. Phylogenies inferred from different sequence-based alignments were relatively more similar ( i.e. , tree topology and branch support) to one another than to secondary structure-aided methods. Finally, conflicting branch support values can be reconciled by properly selecting the alignment method.}, keywords = {Belgium, China, description, Ecphyadophora, Ecphyadophorinae, Ecphyadophoroides, Filenchus, Lelenchus leptosoma, Malenchus cylindricus sp. n., Mexico, Miculenchus salvus, morphology, Morphometrics, taxonomy, Tenunemellus sheri}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003177}, author = {Qing, Xue and Pereira, Tiago J. and Slos, Dieter and Couvreur, Marjolein and Bert, Wim} } @article {Cortada2019, title = {Integration of nematology as a training and research discipline in sub-Saharan Africa: progress and prospects}, journal = {Nematology}, year = {2019}, pages = {1{\textendash}21}, abstract = {Within sub-Saharan Africa (SSA), although nematodes are viewed among the most important threats to crop production and food security, the presence of trained nematologists working within this discipline has traditionally been viewed as scarce. The few research studies concerning this subject address this topic from a country or sub-regional perspective and generally portray nematology as {\textquoteleft}insufficient{\textquoteright}. Over the past two decades, a few initiatives have been instrumental in building greater nematology expertise. For the first time a structured survey was undertaken, involving interviews with individuals from SSA that were (or currently are) involved in nematology training programmes, research, national extension services or in African universities. This paper provides evidence of the positive impact of various initiatives and shows an increase in the number of available nematology positions, together with high rates of graduates that return home to occupy qualified positions. Our findings will help researchers, policy makers and donors to identify areas requiring support to increase the promotion of nematology in SSA and to make an impact for end-users.}, keywords = {academic curricula, capacity building, education, eumaine, extension services, imanema, pinc, tertiary institutions}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003291}, author = {Cortada, Laura and Dehennin, Inge and Bert, Wim and Coyne, Danny} } @article {Qing2019, title = {Phylogeography and Molecular Species Delimitation of Pratylenchus capsici n. Sp., a New Root-Lesion Nematode in Israel on Pepper (Capsicum annuum)}, journal = {Phytopathology}, volume = {109}, number = {5}, year = {2019}, pages = {847{\textendash}858}, keywords = {Arava rift, Capsicum annuum, Nematoda, new species, Pepper, Pratylenchus, tylenchomorpha}, issn = {0031949X}, doi = {10.1094/PHYTO-09-18-0324-R}, author = {Qing, Xue and Bert, Wim and Gamliel, Abraham and Bucki, Patricia and Duvrinin, Svetlana and Alon, Tamar and Miyara, Sigal Braun} } @article {Nguyen2019, title = {Molecular and morphological characterisation of a new root-lesion nematode, Pratylenchus horti n. sp. (Tylenchomorpha: Pratylenchidae), from Ghent University Botanical Garden}, journal = {Nematology}, volume = {21}, number = {7}, year = {2019}, pages = {739{\textendash}752}, abstract = {Root-lesion nematodes, Pratylenchus spp., are one of the most important nematode groups in economic terms. The combination of morphological analyses and molecular analyses based on D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA regions supported the establishment of a new Pratylenchus species, making a total of 103 valid Pratylenchus species. The females of P. horti n. sp. are characterised by the following traits: low labial region with two annuli continuous to the body, en face form belonging to group II sensu Corbett \& Clark (1983) with submedian triangular-shaped segments fused with the oral disc and separated from the lateral segments, lateral field with four incisures at vulval level and lacking areolation, robust stylet 15-17 μ m long with rounded knobs, and subcylindrical tail with smooth tail tip. The males are largely similar to the females but differ from the females by the partially areolated lateral field, slightly ventrally arcuate and weakly cephalated spicules (15-19 μ m), and ventrally curved elongate conical tail with a poorly protruding, crenate bursa. The new species was recovered from soil and root samples from the rhizosphere of Hedychium greenii growing in the Botanical Garden, Ghent University, Belgium.}, keywords = {28S, Belgium, cluster analysis, coi, d2-d3, exotic plant, Ginger lily, Hedychium greenii, its, morphology, Morphometrics, mtDNA, new species, plant-parasitic nematode, rDNA, taxonomy, web-based key}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003249}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Nguyen2019a, title = {Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae)}, journal = {Journal of Nematology}, volume = {51}, number = {1}, year = {2019}, pages = {1{\textendash}20}, abstract = {During a survey in the Botanical garden of Ghent University, a new species Rotylenchus rhomboides n. sp. and a population of Rotylenchus buxophilus were found. Rotylenchus rhomboides n. sp. is characterized by the presence of a rhomboid-like widening of the mid-ridge of lateral field at the level of vulva, a feature previously unknown within the genus. The population of the new species, composed only by females, has a rounded labial region with 4 to 5 annuli, robust stylet (31{\textendash}37μm long), short dorsal esophageal gland (9{\textendash}19μm) overlap of the intestine, vulva located slightly posterior to mid-body, and hemispherical or rounded tail shape with large phasmids located 3 to 5 annuli anterior to the level of anus. The hierarchical cluster analysis based on morphological features indicated that the new species closely resembles R. corsicus, R. gracilidens, and R. rugatocuticulatus. The DNA analyses of the D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA sequences of Rotylenchus rhomboides n. sp. show a close relationship with R. buxophilus, R. goodeyi, R. laurentinus, R. pumilus, and R. incultus, all of which can also be differentiated from the new species by morphological features. The combination of morphological, morphometric, and molecular characteristics confirmed the new species and the first report of R. buxophilus on yam (Dioscorea tokoro) in Belgium.}, keywords = {28S{\textendash}D2D3, coi, Dioscorea tokoro, Hierarchical cluster, its, morphology, Musa basjoo, phylogeny, Plant-parasitic, Rotylenchus, Systematic, taxonomy}, issn = {0022300X}, doi = {10.21307/jofnem-2019-023}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Nguyen2019b, title = {First Report of Scutellonema brachyurus (Steiner, 1938) Andrassy, 1958 and Occurrence of Meloidogyne incognita (Kofoid \& White, 1919) Chitwood, 1949 in Belgium}, journal = {Journal of Nematology}, volume = {51}, number = {2018}, year = {2019}, pages = {1{\textendash}6}, keywords = {by, com, e-mail, global warming, gmail, Hedychium greenii, mtDNA, Musa basjoo, nad5, this paper was edited, tqphap}, issn = {0022-300X}, doi = {10.21307/jofnem-2019-062}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Wang2019, title = {Aphelenchoides medicagus n. sp. (Tylenchina: Aphelenchoididae) found in Medicago sativa imported into China from the USA}, journal = {Nematology}, volume = {21}, year = {2019}, pages = {709{\textendash}723}, abstract = {Aphelenchoides medicagus n. sp. isolated in Ningbo Port, P.R. China, from dried leaves and shoots of Medicago sativa imported from the USA, is described. It is characterised by a lateral field with four lines (three bands), stylet 9.0-12.0 μ m long, excretory pore situated at same level as, or slightly posterior, to the nerve ring, vagina sclerotised, female vulval flap absent, post-uterine sac short, spicules 9.2-14.2 μ m long (median curved line), rosethorn-shaped, apex and rostrum rounded, poorly developed, female and male tail conical with terminus bearing star-like processes. The new species belongs to the Group 3 category of Aphelenchoides species sensu Shahina. Phylogenetic analyses based on 18S, ITS and 28S D2-D3 of rDNA and mtCOI sequences confirmed its status as a new species and closely related to A. besseyi and A. fujianensis .}, keywords = {alfalfa, description, lucerne, molecular, morphology, Morphometrics, new species, phylogeny, plant-parasitic nematode, quarantine, SEM, taxonomy}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003247}, author = {Wang, Zhen and Bert, Wim and Gu, Jianfeng and Couvreur, Marjolein and Li, Hongmei} } @article {Niragire2019, title = {First Report of Potato Cyst Nematode (Globodera rostochiensis) Infecting Potato (Solanum tuberosum L.) in Rwanda}, journal = {Plant Disease}, volume = {409}, year = {2019}, pages = {19{\textendash}21}, isbn = {1568541100003}, issn = {0191-2917}, doi = {10.1094/pdis-04-19-0891-pdn}, author = {Niragire, Ildephonse and Couvreur, Marjolein and Karssen, Gerrit and Uwumukiza, Beatrice and Bert, Wim} } @article {Claeys2019a, title = {Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes}, journal = {Nematology}, volume = {22}, year = {2019}, pages = {1{\textendash}8}, abstract = {Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation, and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii . A~comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore, SPRF fixation is proposed as an affordable, relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes.}, keywords = {a, a hurdle that needs, at the same time, cuticle, eggshell, globodera rostochiensis, heterodera schachtii, in their early life, nematodes are protected by, one would like to, robust eggshell, second-stage juvenile, stages, the eggshell constitutes, to be overcome if, transmission electron microscopy}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003287}, author = {Claeys, Myriam and Handayani, Nurul Dwi and Yushin, Vladimir V. and Lestari, Prabowo and Dikin, Antarjo and Helder, Johannes and Bert, Wim} } @article {ISI:000499274300001, title = {Widespread prevalence but contrasting patterns of intragenomic rRNA polymorphisms in nematodes: Implications for phylogeny, species delimitation and life history inference}, journal = {Molecular Ecology Resources}, volume = {20}, number = {1}, year = {2020}, month = {jan}, pages = {318{\textendash}332}, publisher = {WILEY}, type = {Article}, address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA}, abstract = {Ribosomal RNA genes have long been a favoured locus in phylogenetic and metabarcoding studies. Within a genome, rRNA loci are organized as tandem repeated arrays and the copies are homogenized through the process of concerted evolution. However, some level of rRNA variation (intragenomic polymorphism) is known to persist and be maintained in the genomes of many species. In nematode worms, the extent of rRNA polymorphism (RP) across species and the evolutionary and life history factors that contribute to the maintenance of intragenomic RP is largely unknown. Here, we present an extensive analysis across 30 terrestrial nematode species representing a range of free-living and parasitic taxa isolated worldwide. Our results indicate that RP is common and widespread, ribosome function appears to be maintained despite mutational changes, and intragenomic variants are stable in the genome and neutrally evolving. However, levels of variation were varied widely across rRNA locus and species, with some taxa observed to lack RP entirely. Higher levels of RP were significantly correlated with shorter generation time and high reproductive rates, and population-level factors may play a role in the geographic and phylogenetic structuring of rRNA variants observed in genera such as Rotylenchulus and Pratylenchus. Although RP did not dramatically impact the clustering and recovery of taxa in mock metabarcoding analyses, the present study has significant implications for global biodiversity estimates of nematode species derived from environmental rRNA amplicon studies, as well as our understanding of the evolutionary and ecological factors shaping genetic diversity across the nematode Tree of Life.}, doi = {10.1111/1755-0998.13118}, author = {Qing, Xue and Bik, Holly and Yergaliyev, Timur M and Gu, Jianfeng and Fonderie, Pamela and Brown-Miyara, Sigal and Szitenberg, Amir and Bert, Wim} } @article {Claeys2019, title = {Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes}, journal = {Nematology}, volume = {22}, year = {2020}, pages = {103{\textendash}110}, isbn = {1568541100003}, doi = {10.1163/15685411-00003287}, author = {Claeys, Myriam and Dwi Handayani, Nurul and Yushin, Vladimir V. and Lestari, Prabowo and Dikin, Antarjo and Helder, Johannes and Bert, Wim} } @article {722, title = {Description of Malenchus sexlineatus n. sp., new records of three known species of Malenchus andrassy, 1968 (Nematoda: Tylenchidae) and notes on amphidial aperture development}, journal = {NematologyNematology}, volume = {18}, year = {2016}, note = {Times Cited: 02}, month = {2016}, pages = {155-174}, abstract = {A new species, Malenchus sexlineatus n. sp., discovered from the Philippines, is described based on morphological and molecular data. The new species is unusual in the genus by having six lateral lines. Malenchus sexlineatus n. sp. is distinguished from M. williamsi, the only other species in the genus with six lateral lines (based on currently available SEM data), by a shorter body of 278 (270-288) vs 452 (425-495) mu m, shorter stylet (7.0 (6.2-7.5) vs 11-12 mu m), narrower annulations (0.8 (0.7-0.8) vs 1.2-1.6 mu m), lateral field comprising one elevated ridge in LM vs six well-separated incisures (resembling the lateral lines in Cephalenchus) in LM, the presence of S-shaped vs straight amphidial apertures, and vulval flaps absent or only one annuli long vs distinct. By having an exceptionally short body, M. sexlineatus n. sp. comes close to M. parvus, M. bryanti and M. acarayensis. However, there are significant differences in the lateral lines, annuli width and most morphometric ratios. Three known species, namely M. exiguus, M. nanellus and M. pachycephalus, all being first records and first representative from China, are characterised by morphological data. The new species was placed in a robustly supported clade containing two other Malenchus spp. and M. exiguus. Interestingly, M. pressulus was placed in a separate, unresolved phylogenetic position. However, the phylogenetic position of these clades could not be resolved within Tylenchidae. The shapes of the amphidial aperture and fovea within Malenchus are also compared and its possible developmental process is illustrated and discussed.}, isbn = {1388-5545}, author = {Qing, Xue and Sanchez-Monge, Alcides and Janssen, Toon and Couvreur, Marjolein and Bert, Wim} } @article {727, title = {Description of Phasmarhabditis californica n. sp and first report of P. papillosa (Nematoda: Rhabditidae) from invasive slugs in the USA}, journal = {NematologyNematology}, volume = {18}, year = {2016}, note = {Times Cited: 02}, month = {2016}, pages = {175-193}, abstract = {A new species of Phasmarhabditis and the known species P. papillosa were isolated from cadavers of invasive slugs in California. Phasmarhabditis papillosa is the type of the genus and has not previously been reported from the Americas. Both species are characterised based on morphology, morphometrics and molecular data. Molecular phylogenies were inferred from concatenated DNA sequence alignments of nearly complete SSU and the D2-D3 domains of the LSU rDNA. Phasmarhabditis californica n. sp. is characterised by a robust body, mature egg-laying specimens almost spindle-shaped when relaxed, 1.5 (1.3-1.8) mm long, tapering to a bluntly rounded anterior end with stoma about as long as lip region diam., six inner labial papillae and four outer cephalic papillae, pharynx with rounded to pyriform basal bulb, vulva located halfway along the body, hermaphroditic, didelphic, amphidelphic, and short, conoid tail constricted at one-third its length with prominent phasmids. Not a single male was found among five strains. Phasmarhabditis papillosa is gonochoristic and has a longer isthmus, pyriform basal bulb and longer, dome-shaped spicate female tail constricted halfway along its length. Sequence analysis revealed unambiguous autapomorphies for P. papillosa and P. californica n. sp. Phylogenetic analyses placed these two species in a monophyletic clade comprising Phasmarhabditis species and other gastropod-parasitic taxa. Morphology, genetic distance, reproductive strategy and nucleotide autapomorphies support the new taxon.}, isbn = {1388-5545}, author = {Tandingan De Ley, Irma and Holovachov, Oleksandr and Mc Donnell, Rory J. and Bert, Wim and Paine, Timothy D. and De Ley, Paul} } @article {711, title = {Cryo-fixation and associated developments in transmission electron microscopy: a cool future for nematology}, journal = {NematologyNematology}, volume = {18}, year = {2016}, note = {Times Cited: 01}, month = {2016}, pages = {1-14}, isbn = {1388-5545}, author = {Bert, Wim and Slos, Dieter and Leroux, Olivier and Claeys, Myriam} } @article {408, title = {First report, morphological and molecular characterization of Xiphinema elongatum and X. pachtaicum (Nematoda, Longidoridae) from Ethiopia}, journal = {ZookeysZookeys}, number = {489}, year = {2015}, note = {Times Cited: 0}, month = {2015}, pages = {1-13}, isbn = {1313-2989}, author = {Getaneh, Gezahegne and Bert, Wim and Decraemer, Wilfrida} } @article {414, title = {Laimaphelenchus hyrcanus n. sp. (Nematoda: Aphelenchoididae), a new species from northern Iran}, journal = {ZootaxaZootaxa}, volume = {3915}, number = {4}, year = {2015}, note = {Times Cited: 0}, month = {Feb 5}, pages = {591-600}, isbn = {1175-5326}, author = {Miraeiz, Esmaeil and Heydari, Ramin and Maafi, Zahra Tanha and Bert, Wim} } @article {71, title = {Temporal dynamics in nematode biodiversity and community structure at an experimental open ocean aquaculture site, Gulf of Maine, USA}, journal = {Russian Journal of NematologyRussian Journal of Nematology}, volume = {20}, number = {2}, year = {2012}, note = {Times Cited: 0}, month = {2012}, pages = {127-140}, isbn = {0869-6918}, author = {Abebe, Eyualem and Joseph, Matthew and Bert, Wim} } @article {27, title = {Nematodes from terrestrial, freshwater and brackish water habitats in Belgium: an updated list with special emphasis on compost nematodes}, journal = {ZootaxaZootaxa}, volume = {3765}, number = {2}, year = {2014}, note = {Times Cited: 0}, month = {Feb 17}, pages = {143-160}, isbn = {1175-5326; 1175-5334}, author = {Steel, Hanne and Coomans, August and Decraemer, Wilfrida and Moens, Tom and Bert, Wim} } @article {32, title = {Revision of Acrochordonoposthia Reisinger, 1924 (Rhabditophora, Typhloplanidae, Protoplanellinae) with the description of one new species}, journal = {ZootaxaZootaxa}, volume = {3790}, number = {1}, year = {2014}, note = {Times Cited: 0}, month = {Apr 16}, pages = {36-50}, isbn = {1175-5326; 1175-5334}, author = {Houben, Albrecht M. and Proesmans, Willem and Bert, Wim and Artois, Tom J.} }