@article {Cortada2019, title = {Integration of nematology as a training and research discipline in sub-Saharan Africa: progress and prospects}, journal = {Nematology}, year = {2019}, pages = {1{\textendash}21}, abstract = {Within sub-Saharan Africa (SSA), although nematodes are viewed among the most important threats to crop production and food security, the presence of trained nematologists working within this discipline has traditionally been viewed as scarce. The few research studies concerning this subject address this topic from a country or sub-regional perspective and generally portray nematology as {\textquoteleft}insufficient{\textquoteright}. Over the past two decades, a few initiatives have been instrumental in building greater nematology expertise. For the first time a structured survey was undertaken, involving interviews with individuals from SSA that were (or currently are) involved in nematology training programmes, research, national extension services or in African universities. This paper provides evidence of the positive impact of various initiatives and shows an increase in the number of available nematology positions, together with high rates of graduates that return home to occupy qualified positions. Our findings will help researchers, policy makers and donors to identify areas requiring support to increase the promotion of nematology in SSA and to make an impact for end-users.}, keywords = {academic curricula, capacity building, education, eumaine, extension services, imanema, pinc, tertiary institutions}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003291}, author = {Cortada, Laura and Dehennin, Inge and Bert, Wim and Coyne, Danny} } @article {Qing2019, title = {Phylogeography and Molecular Species Delimitation of Pratylenchus capsici n. Sp., a New Root-Lesion Nematode in Israel on Pepper (Capsicum annuum)}, journal = {Phytopathology}, volume = {109}, number = {5}, year = {2019}, pages = {847{\textendash}858}, keywords = {Arava rift, Capsicum annuum, Nematoda, new species, Pepper, Pratylenchus, tylenchomorpha}, issn = {0031949X}, doi = {10.1094/PHYTO-09-18-0324-R}, author = {Qing, Xue and Bert, Wim and Gamliel, Abraham and Bucki, Patricia and Duvrinin, Svetlana and Alon, Tamar and Miyara, Sigal Braun} } @article {Nguyen2019, title = {Molecular and morphological characterisation of a new root-lesion nematode, Pratylenchus horti n. sp. (Tylenchomorpha: Pratylenchidae), from Ghent University Botanical Garden}, journal = {Nematology}, volume = {21}, number = {7}, year = {2019}, pages = {739{\textendash}752}, abstract = {Root-lesion nematodes, Pratylenchus spp., are one of the most important nematode groups in economic terms. The combination of morphological analyses and molecular analyses based on D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA regions supported the establishment of a new Pratylenchus species, making a total of 103 valid Pratylenchus species. The females of P. horti n. sp. are characterised by the following traits: low labial region with two annuli continuous to the body, en face form belonging to group II sensu Corbett \& Clark (1983) with submedian triangular-shaped segments fused with the oral disc and separated from the lateral segments, lateral field with four incisures at vulval level and lacking areolation, robust stylet 15-17 μ m long with rounded knobs, and subcylindrical tail with smooth tail tip. The males are largely similar to the females but differ from the females by the partially areolated lateral field, slightly ventrally arcuate and weakly cephalated spicules (15-19 μ m), and ventrally curved elongate conical tail with a poorly protruding, crenate bursa. The new species was recovered from soil and root samples from the rhizosphere of Hedychium greenii growing in the Botanical Garden, Ghent University, Belgium.}, keywords = {28S, Belgium, cluster analysis, coi, d2-d3, exotic plant, Ginger lily, Hedychium greenii, its, morphology, Morphometrics, mtDNA, new species, plant-parasitic nematode, rDNA, taxonomy, web-based key}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003249}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Nguyen2019a, title = {Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae)}, journal = {Journal of Nematology}, volume = {51}, number = {1}, year = {2019}, pages = {1{\textendash}20}, abstract = {During a survey in the Botanical garden of Ghent University, a new species Rotylenchus rhomboides n. sp. and a population of Rotylenchus buxophilus were found. Rotylenchus rhomboides n. sp. is characterized by the presence of a rhomboid-like widening of the mid-ridge of lateral field at the level of vulva, a feature previously unknown within the genus. The population of the new species, composed only by females, has a rounded labial region with 4 to 5 annuli, robust stylet (31{\textendash}37μm long), short dorsal esophageal gland (9{\textendash}19μm) overlap of the intestine, vulva located slightly posterior to mid-body, and hemispherical or rounded tail shape with large phasmids located 3 to 5 annuli anterior to the level of anus. The hierarchical cluster analysis based on morphological features indicated that the new species closely resembles R. corsicus, R. gracilidens, and R. rugatocuticulatus. The DNA analyses of the D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA sequences of Rotylenchus rhomboides n. sp. show a close relationship with R. buxophilus, R. goodeyi, R. laurentinus, R. pumilus, and R. incultus, all of which can also be differentiated from the new species by morphological features. The combination of morphological, morphometric, and molecular characteristics confirmed the new species and the first report of R. buxophilus on yam (Dioscorea tokoro) in Belgium.}, keywords = {28S{\textendash}D2D3, coi, Dioscorea tokoro, Hierarchical cluster, its, morphology, Musa basjoo, phylogeny, Plant-parasitic, Rotylenchus, Systematic, taxonomy}, issn = {0022300X}, doi = {10.21307/jofnem-2019-023}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Nguyen2019b, title = {First Report of Scutellonema brachyurus (Steiner, 1938) Andrassy, 1958 and Occurrence of Meloidogyne incognita (Kofoid \& White, 1919) Chitwood, 1949 in Belgium}, journal = {Journal of Nematology}, volume = {51}, number = {2018}, year = {2019}, pages = {1{\textendash}6}, keywords = {by, com, e-mail, global warming, gmail, Hedychium greenii, mtDNA, Musa basjoo, nad5, this paper was edited, tqphap}, issn = {0022-300X}, doi = {10.21307/jofnem-2019-062}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Wang2019, title = {Aphelenchoides medicagus n. sp. (Tylenchina: Aphelenchoididae) found in Medicago sativa imported into China from the USA}, journal = {Nematology}, volume = {21}, year = {2019}, pages = {709{\textendash}723}, abstract = {Aphelenchoides medicagus n. sp. isolated in Ningbo Port, P.R. China, from dried leaves and shoots of Medicago sativa imported from the USA, is described. It is characterised by a lateral field with four lines (three bands), stylet 9.0-12.0 μ m long, excretory pore situated at same level as, or slightly posterior, to the nerve ring, vagina sclerotised, female vulval flap absent, post-uterine sac short, spicules 9.2-14.2 μ m long (median curved line), rosethorn-shaped, apex and rostrum rounded, poorly developed, female and male tail conical with terminus bearing star-like processes. The new species belongs to the Group 3 category of Aphelenchoides species sensu Shahina. Phylogenetic analyses based on 18S, ITS and 28S D2-D3 of rDNA and mtCOI sequences confirmed its status as a new species and closely related to A. besseyi and A. fujianensis .}, keywords = {alfalfa, description, lucerne, molecular, morphology, Morphometrics, new species, phylogeny, plant-parasitic nematode, quarantine, SEM, taxonomy}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003247}, author = {Wang, Zhen and Bert, Wim and Gu, Jianfeng and Couvreur, Marjolein and Li, Hongmei} } @article {Niragire2019, title = {First Report of Potato Cyst Nematode (Globodera rostochiensis) Infecting Potato (Solanum tuberosum L.) in Rwanda}, journal = {Plant Disease}, volume = {409}, year = {2019}, pages = {19{\textendash}21}, isbn = {1568541100003}, issn = {0191-2917}, doi = {10.1094/pdis-04-19-0891-pdn}, author = {Niragire, Ildephonse and Couvreur, Marjolein and Karssen, Gerrit and Uwumukiza, Beatrice and Bert, Wim} } @article {Claeys2019a, title = {Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes}, journal = {Nematology}, volume = {22}, year = {2019}, pages = {1{\textendash}8}, abstract = {Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation, and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii . A~comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore, SPRF fixation is proposed as an affordable, relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes.}, keywords = {a, a hurdle that needs, at the same time, cuticle, eggshell, globodera rostochiensis, heterodera schachtii, in their early life, nematodes are protected by, one would like to, robust eggshell, second-stage juvenile, stages, the eggshell constitutes, to be overcome if, transmission electron microscopy}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003287}, author = {Claeys, Myriam and Handayani, Nurul Dwi and Yushin, Vladimir V. and Lestari, Prabowo and Dikin, Antarjo and Helder, Johannes and Bert, Wim} } @article {Singh2019, title = {Survey of slug-parasitic nematodes in East and West Flanders , Belgium and description of Angiostoma gandavensis n. sp. (Nematoda: Angiostomidae) from arionid slugs}, journal = {Journal of Helminthology}, year = {2019}, pages = {1{\textendash}11}, doi = {https://doi.org/10.1017/ S0022149X19000105}, author = {Singh, P R and Couvreur, M and Decraemer, W and Bert, W} }