@article {Claeys2019a,
	title = {Self-pressurised rapid freezing, a time-efficient and affordable cryo-fixation method for ultrastructural observations on unhatched cyst nematodes},
	journal = {Nematology},
	volume = {22},
	year = {2019},
	pages = {1{\textendash}8},
	abstract = {Ultrastructural analysis of the early development of nematodes is hampered by the impermeability of the eggshell to most commonly used fixatives. High-pressure freezing (HPF), a physical cryo-fixation method, facilitates a fast rate of fixation, and by using this method the issue of the uneven delivery of fixative is circumvented. Although HPF results in a superior preservation of the fine structure, the equipment costs impede a wider application of this method. Self-pressurised rapid freezing (SPRF) is an alternative low-cost cryo-fixation method, and its usefulness was evaluated in an ultrastructural study of the eggshell and the cuticle of unhatched second-stage juveniles (J2) of Globodera rostochiensis and Heterodera schachtii . A~comparison with conventional (chemical) fixation demonstrates that SPRF fixation results in a remarkably well-preserved ultrastructure of the entire egg including both the eggshell and the cellular details of the unhatched J2. Therefore, SPRF fixation is proposed as an affordable, relatively easy-to-use and time-efficient technique to study the ultrastructure of unhatched J2 and eggs of nematodes.},
	keywords = {a, a hurdle that needs, at the same time, cuticle, eggshell, globodera rostochiensis, heterodera schachtii, in their early life, nematodes are protected by, one would like to, robust eggshell, second-stage juvenile, stages, the eggshell constitutes, to be overcome if, transmission electron microscopy},
	isbn = {1568541100003},
	issn = {1388-5545},
	doi = {10.1163/15685411-00003287},
	author = {Claeys, Myriam and Handayani, Nurul Dwi and Yushin, Vladimir V. and Lestari, Prabowo and Dikin, Antarjo and Helder, Johannes and Bert, Wim}
}
@article {171,
	title = {Cuticular structure of three<i> Bunonema</i> species and first description of the male of <i>B. penard</i>i Stefanski, 1914 (Nematoda : Bunonematidae)},
	journal = {NematologyNematology},
	volume = {6},
	year = {2004},
	note = {Times Cited: 0 Article English Borgonie, G State Univ Ghent, Dept Biol, Nematol Sect, KL Ledeganckstr 35, B-9000 Ghent, Belgium Cited References Count: 38 889AI BRILL ACADEMIC PUBLISHERS PLANTIJNSTRAAT 2, P O BOX 9000, 2300 PA LEIDEN, NETHERLANDS LEIDEN Part 4},
	pages = {473-484},
	abstract = {This study reanalyses some morphological and ultrastructural aspects of three Bunonema species. Specimens of B. penardi, B. hessi and B.franzi were studied using light microscopy and SEM. Bunonema franzi was also studied by TEM. The male of B. penardi is described, this being the first record of males in the genus Bunonema. Additional morphometric data on the females of this species are presented. SEM pictures show details of the deviant amphid position within the Bunonematidae. In contrast to earlier reports, our TEM results show that the evagination of the cuticle in ridges and tubercles is due to the thickening of the median zone of the cuticle. Ultrastructural details of the species-specific polygonal mesh network on the right body side are revealed. The rods that constitute this network lie above the epicuticle and are therefore extracuticular},
	keywords = {BODY WALL, cuticle, FINE-STRUCTURE, GENUS, light microscopy, microscopy, morphology, Nematoda, PROTEIN, RHABDITIDA, SEM, STOMA, TEM, tubercle, ultrastructure, YOLK},
	isbn = {1388-5545},
	author = {Van Gansbeke, R. and Bert, W. and Govaerts, A. and Claeys, M. and Van Driessche, R. and Manhout, J. and Borgonie, G.}
}
