@article {412,
	title = {Protozoan cysts act as survival niche and protective shelter for foodborne pathogenic bacteria},
	journal = {Applied and Environmental MicrobiologyApplied and Environmental Microbiology},
	year = {Submitted},
	author = {Lambrecht, E. and Bar{\'e}, J. and Chavatte, N. and Bert, W. and Sabbe, K. and Houf, K.}
}
@article {413,
	title = {Transmission electron microscopy sample preparation protocols for ultrastructural study of cysts of free-living protozoa},
	journal = {BiotechniquesBiotechniques},
	volume = {58},
	year = {2015},
	pages = {181-188},
	author = {Lambrecht, E. and Bar{\'e}, J. and Claeys, M. and Chavatte, N. and Bert, W. and Sabbe, K. and Houf, K.}
}
@article {47,
	title = {Behavior of <i>Yersinia enterocolitica</i> in the presence of the bacterivorous <i>Acanthamoeba castellanii</i>},
	journal = {Applied and Environmental MicrobiologyApplied and Environmental MicrobiologyApplied and Environmental Microbiology},
	volume = {79},
	number = {20},
	year = {2013},
	note = {ISI Document Delivery No.: 236OHTimes Cited: 0Cited Reference Count: 53Cited References:      Akya A, 2009, FEMS MICROBIOL ECOL, V70, P20, DOI 10.1111/j.1574-6941.2009.00736.x     Alsam S, 2006, J MED MICROBIOL, V55, P689, DOI 10.1099/jmm.0.46497-0     Anacarso I, 2012, J BASIC MICROB, V52, P261, DOI 10.1002/jobm.201100097     Axelsson-Olsson D, 2010, EXP PARASITOL, V126, P59, DOI 10.1016/j.exppara.2009.12.016     Axelsson-Olsson D, 2010, APPL ENVIRON MICROB, V76, P4194, DOI 10.1128/AEM.01219-09     Bare J, 2011, APPL ENVIRON MICROB, V77, P1763, DOI 10.1128/AEM.01756-10     Bare J, 2010, FEMS MICROBIOL ECOL, V74, P371, DOI 10.1111/j.1574-6941.2010.00955.x     Bare J, 2009, APPL ENVIRON MICROB, V75, P1417, DOI 10.1128/AEM.02346-08     Berk SG, 2008, APPL ENVIRON MICROB, V74, P2187, DOI 10.1128/AEM.01214-07     Berry D, 2010, APPL ENVIRON MICROB, V76, P6685, DOI 10.1128/AEM.00644-10     Bottone EJ, 1997, CLIN MICROBIOL REV, V10, P257     Bottone EJ, 1999, MICROBES INFECT, V1, P323, DOI 10.1016/S1286-4579(99)80028-8     Bruggemann H, 2006, CURR OPIN MICROBIOL, V9, P86, DOI 10.1016/j.mib.2005.12.009     CIRILLO JD, 1994, INFECT IMMUN, V62, P3254     Cirillo JD, 1997, INFECT IMMUN, V65, P3759     Cocolin L, 2005, INT J FOOD MICROBIOL, V105, P71, DOI 10.1016/j.ijfoodmicro.2005.05.006     Corsaro D, 2013, EXP PARASITOL, V133, P383, DOI 10.1016/j.exppara.2012.12.016     de Felipe KS, 2008, PLOS PATHOG, V4, DOI 10.1371/journal.ppat.1000117     Douesnard-Malo F, 2011, APPL ENVIRON MICROB, V77, P7640, DOI 10.1128/AEM.00699-11     EFSA, 2013, EFSA J, V11, P3129, DOI DOI 10.2903/J.EFSA.2013.3129     Falcao JP, 2004, J APPL MICROBIOL, V96, P1230, DOI 10.1111/j.1365-2672.2004.02268.x     FARMER JJ, 1992, J CLIN MICROBIOL, V30, P2589     Fredriksson-Ahomaa M, 2006, FEMS IMMUNOL MED MIC, V47, P315, DOI 10.1111/j.1574-695X.2006.00095.x     Gaze WH, 2003, MICROBIAL ECOL, V46, P358, DOI 10.1007/s00248-003-1001-3     Goverde RLJ, 1998, MOL MICROBIOL, V28, P555, DOI 10.1046/j.1365-2958.1998.00816.x     Greub G, 2003, ANN NY ACAD SCI, V990, P628     Greub G, 2004, CLIN MICROBIOL REV, V17, P413, DOI 10.1128/CMR.17.2.413-433.2004     Hausmann K, 2003, PROTISTOLOGY SCHWEIZ     Jackson V, 2007, FOOD CONTROL, V18, P346, DOI 10.1016/j.foodcont.2005.10.018     Khan N., 2009, ACANTHAMOEBA BIOL PA     KING CH, 1988, APPL ENVIRON MICROB, V54, P3023     Koubar M, 2011, FEMS MICROBIOL LETT, V325, P10, DOI 10.1111/j.1574-6968.2011.02402.x     Matz C, 2005, TRENDS MICROBIOL, V13, P302, DOI 10.1016/j.tim.2005.05.009     McCuddin Z, 2006, VET MICROBIOL, V114, P275, DOI 10.1016/j.vetmic.2005.12.004     Nesbakken T, 2003, INT J FOOD MICROBIOL, V80, P231, DOI 10.1016/S0168-1605(02)00165-4     Pickup ZL, 2007, APPL ENVIRON MICROB, V73, P2631, DOI 10.1128/AEM.02061-06     RILEY G, 1989, J CLIN MICROBIOL, V27, P213     Robinson CG, 2006, CELL MICROBIOL, V8, P793, DOI 10.1111/j.1462-5822.2005.00666.x     Rohde JR, 1999, J BACTERIOL, V181, P4198     Siddiqui R, 2012, EXP PARASITOL, V130, P311, DOI 10.1016/j.exppara.2012.01.021     SMITH JJ, 1994, APPL ENVIRON MICROB, V60, P2977     Snelling WJ, 2008, ARCH MICROBIOL, V189, P175, DOI 10.1007/s00203-007-0303-0     SPURR AR, 1969, J ULTRA MOL STRUCT R, V26, P31, DOI 10.1016/S0022-5320(69)90033-1     Tezcan-Merdol D, 2004, APPL ENVIRON MICROB, V70, P3706, DOI 10.1128/AEM.70.6.3706-3714.2004     Thomas V, 2010, FEMS MICROBIOL REV, V34, P231, DOI 10.1111/j.1574-6976.2009.00190.x     Vaerewijck MJM, 2010, J APPL MICROBIOL, V109, P1566, DOI 10.1111/j.1365-2672.2010.04783.x     Vaerewijck MJM, 2011, INT J FOOD MICROBIOL, V147, P105, DOI 10.1016/j.ijfoodmicro.2011.03.015     Vaerewijck MJM, 2012, J FOOD PROTECT, V75, P541, DOI 10.4315/0362-028X.JFP-11-359     Vaerewijck MJM, 2008, APPL ENVIRON MICROB, V74, P5741, DOI 10.1128/AEM.00980-08     Van Damme I, 2010, FOOD MICROBIOL, V27, P158, DOI 10.1016/j.fm.2009.09.011     Van Damme I, 2013, INT J FOOD MICROBIOL, V164, P32, DOI 10.1016/j.ijfoodmicro.2013.03.019     Verhaegen J, 1998, CLIN INFECT DIS, V27, P59, DOI 10.1086/514636     Whitworth T, 2005, INFECT IMMUN, V73, P6668, DOI 10.1128/IAI.73.10.6668-6673.2005Lambrecht, E. Bare, J. Van Damme, I. Bert, W. Sabbe, K. Houf, K.Research Fund of Ghent University (BOF, Ghent, Belgium) [01D07612]This work was supported by the Research Fund of Ghent University (BOF, Ghent, Belgium; grant 01D07612).Amer soc microbiologyWashington},
	month = {Oct},
	pages = {6407-6413},
	type = {Article},
	abstract = {Free-living protozoa play an important role in the ecology and epidemiology of human-pathogenic bacteria. In the present study, the interaction between Yersinia enterocolitica, an important food-borne pathogen, and the free-living amoeba Acanthamoeba castellanii was studied. Several cocultivation assays were set up to assess the resistance of Y. enterocolitica to A. castellanii predation and the impact of environmental factors and bacterial strain-specific characteristics. Results showed that all Y. enterocolitica strains persist in association with A. castellanii for at least 14 days, and associations with A. castellanii en-hanced survival of Yersinia under nutrient-rich conditions at 25 degrees C and under nutrient-poor conditions at 37 degrees C. Amoebae culti-vated in the supernatant of one Yersinia strain showed temperature-and time-dependent permeabilization. Intraprotozoan survival of Y. enterocolitica depended on nutrient availability and temperature, with up to 2.8 log CFU/ml bacteria displaying intracellular survival at 7 degrees C for at least 4 days in nutrient-rich medium. Transmission electron microscopy was performed to locate the Yersinia cells inside the amoebae. As Yersinia and Acanthamoeba share similar ecological niches, this interaction iden-tifies a role of free-living protozoa in the ecology and epidemiology of Y. enterocolitica.},
	keywords = {amebas, campylobacter-jejuni, diversity, domestic refrigerators, free-living protozoa, legionella-pneumophila, mycobacterium-avium, polyphaga, salmonella-enterica, SURVIVAL},
	isbn = {0099-2240},
	author = {Lambrecht, E. and Bar{\'e}, J. and Van Damme, I. and Bert, W. and Sabbe, K. and Houf, K.}
}
@article {20,
	title = {Transmission electron microscopy sample preparation protocols for ultrastructural study of cysts of free-living protozoa},
	journal = {BiotechniquesBiotechniques},
	year = {2015},
	author = {Lambrecht, E. and Bar{\'e}, J. and Bert, W. and Sabbe, K. and Claeys, M. and Chavatte, N. and Houf, K.}
}
