@article {Claeys2017, title = {Self-Pressurised Rapid Freezing (SPRF): an easy-to-use and low-cost alternative cryo-fixation method for nematodes}, journal = {Nematology}, volume = {19}, number = {8}, year = {2017}, pages = {871{\textendash}881}, keywords = {acrobeles complexus, Caenorhabditis elegans, for over 50 years, freeze substitution, has been a crucial, high-pressure freezing, immunogold labelling, major, method for studying tissues, msp, sperm protein, spermatozoa, TEM, transmission electron microscopy}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003093}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003093}, author = {Claeys, Myriam and Yushin, Vladimir V. and Leunissen, Jan L.M. and Claeys, Jef and Bert, Wim} }