@article {Godjo2018, title = {Steinernema kandii n. sp. (Rhabditida: Steinernematidae), a~new~entomopathogenic nematode from northern Benin}, journal = {Nematology}, year = {2018}, pages = {1{\textendash}22}, keywords = {1934, bicornutum -group, d2-d3, description, entomopathogenic nematodes, epn, have been, its, molecular, morphology, Morphometrics, new species, of the family, phylogeny, RHABDITIDA, steinernematidae filipjev, taxonomy, xenorhabdus indica}, isbn = {1568541100003}, issn = {1388-5545}, doi = {10.1163/15685411-00003201}, url = {http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003201}, author = {Godjo, Anique and Afouda, Leonard and Baimey, Hugues and Couvreur, Marjolein and Zadji, Lionel and Houssou, Gladys and Bert, Wim and Willems, Anne and Decraemer, Wilfrida} } @article {ISI:000449120200008, title = {Hidden diversity in mushrooms explored: A new nematode species, Neodiplogaster unguispiculata sp n. (Rhabditida, Diplogastridae), with a key to the species of Neodiplogaster}, journal = {Zoologischer Anzeiger}, volume = {276}, year = {2018}, month = {sep}, pages = {71{\textendash}85}, publisher = {ELSEVIER GMBH, URBAN \& FISCHER VERLAG}, type = {Article}, address = {OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY}, abstract = {Neodiplogaster unguispiculata n. sp. is based on morphological and molecular data. N. unguispiculata sp. n. was isolated from Chondrostereum purpureum in Lappersheide, Tessenderlo, Belgium and cultured on potato dextrose agar seeded with fungi. N. unguispiculata sp. n. is characterised by a unique combination of the following traits: the morphology of the spicules that are distally claw-like bending ventrally, the shape of the gubernaculum that envelope the spicules, the well-developed peloderan bursa with a notched tip, a bursa spanning from anterior the cloaca to tail tip, and by the long, filiform female tail. N. unguispiculata sp. n. shows stomatal dimorphism. Phylogenetic analyses shows that monophyly of Neodiplogaster is maximally supported by both maximum likelihood and Bayesian interference methods. Various species delimitation methods appear to produce a variety of different species hypotheses in Neodiplogaster. Our study shows that molecular can support different species hypotheses. Finally, a key to the species of Neodiplogaster is provided. (C) 2018 Elsevier GmbH. All rights reserved.}, keywords = {Evolution, Neodi, phylogeny, systematics, taxonomy}, issn = {0044-5231}, doi = {10.1016/j.jcz.2018.07.004}, author = {Slos, Dieter and Couvreur, Marjolein and Bert, Wim} } @article {Slos2018, title = {Description of Myolaimus mycophilus Slos \& Bert sp. n. (Rhabditida: Myolaimidae)}, journal = {Nematology}, volume = {20}, number = {2}, year = {2018}, pages = {175{\textendash}186}, abstract = {Copyright {\textcopyright} 2018 by Koninklijke Brill NV, Leiden, The Netherlands. Myolaimus mycophilus Slos \& Bert sp. n. was isolated from mushrooms from different locations in Belgium and cultured on nutrient agar seeded with Escherichia coli OP50. Myolaimus mycophilus sp. n. is characterised by a unique combination of the following characters: adults with lateral fields not differentiated, buccal cavity triangular, cheilostom anisomorphic, female with a post-vulval uterine sac as long as the corresponding body diam., wrinkled eggshell, male with seven pairs of genital papillae, cloacal aperture covered by a trapezoidal cloacal flap and with a single ventral papilla at its base. The new species and M. goodeyorum Andr{\'a}ssy, 1984 were molecularly characterised by sequencing the 18S and D2-D3 28S expansion segments of rDNA. Phylogenetic relationships were reconstructed based on concatenated analyses and the phylogenetic placement of Myolaimus, an intriguing genus within the Rhabditida, is discussed.}, keywords = {Belgium, fungi, Ganoderma applanatum, Laetiporus sulphureus, molecular, morphology, Morphometrics, Myolaimus goodeyorum, new species, phylogeny, taxonomy, Trametes}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003134}, author = {Slos, Dieter and Couvreur, Marjolein and Bert, Wim} } @article {Qing2018a, title = {A new species of Malenchus (Nematoda: Tylenchomorpha) with an updated phylogeny of the Tylenchidae}, journal = {Nematology}, volume = {20}, number = {9}, year = {2018}, pages = {815{\textendash}836}, abstract = {The family Tylenchidae is one of most abundant and diverse nematode groups found in soil habitats. In this study, a new species named Malenchus cylindricus sp. n. is described based on light and scanning electron microscopy and molecular data. The new species is characterised by elongate-cylindrical vulva-anus body shape and a narrow annulation. We updated the phylogeny of Tylenchidae by including molecular data for the rare genera Miculenchus and Tenunemellus and by improving the morphological characterisation of the genus Lelenchus . Additionally, we compared the effect of alignment methods on the tree topologies and branch support values. The results suggest that the position of subfamily Ecphyadophorinae within Tylenchidae needs to be further revised. Phylogenies inferred from different sequence-based alignments were relatively more similar ( i.e. , tree topology and branch support) to one another than to secondary structure-aided methods. Finally, conflicting branch support values can be reconciled by properly selecting the alignment method.}, keywords = {Belgium, China, description, Ecphyadophora, Ecphyadophorinae, Ecphyadophoroides, Filenchus, Lelenchus leptosoma, Malenchus cylindricus sp. n., Mexico, Miculenchus salvus, morphology, Morphometrics, taxonomy, Tenunemellus sheri}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003177}, author = {Qing, Xue and Pereira, Tiago J. and Slos, Dieter and Couvreur, Marjolein and Bert, Wim} } @article {Nguyen2019, title = {Molecular and morphological characterisation of a new root-lesion nematode, Pratylenchus horti n. sp. (Tylenchomorpha: Pratylenchidae), from Ghent University Botanical Garden}, journal = {Nematology}, volume = {21}, number = {7}, year = {2019}, pages = {739{\textendash}752}, abstract = {Root-lesion nematodes, Pratylenchus spp., are one of the most important nematode groups in economic terms. The combination of morphological analyses and molecular analyses based on D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA regions supported the establishment of a new Pratylenchus species, making a total of 103 valid Pratylenchus species. The females of P. horti n. sp. are characterised by the following traits: low labial region with two annuli continuous to the body, en face form belonging to group II sensu Corbett \& Clark (1983) with submedian triangular-shaped segments fused with the oral disc and separated from the lateral segments, lateral field with four incisures at vulval level and lacking areolation, robust stylet 15-17 μ m long with rounded knobs, and subcylindrical tail with smooth tail tip. The males are largely similar to the females but differ from the females by the partially areolated lateral field, slightly ventrally arcuate and weakly cephalated spicules (15-19 μ m), and ventrally curved elongate conical tail with a poorly protruding, crenate bursa. The new species was recovered from soil and root samples from the rhizosphere of Hedychium greenii growing in the Botanical Garden, Ghent University, Belgium.}, keywords = {28S, Belgium, cluster analysis, coi, d2-d3, exotic plant, Ginger lily, Hedychium greenii, its, morphology, Morphometrics, mtDNA, new species, plant-parasitic nematode, rDNA, taxonomy, web-based key}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003249}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Nguyen2019a, title = {Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae)}, journal = {Journal of Nematology}, volume = {51}, number = {1}, year = {2019}, pages = {1{\textendash}20}, abstract = {During a survey in the Botanical garden of Ghent University, a new species Rotylenchus rhomboides n. sp. and a population of Rotylenchus buxophilus were found. Rotylenchus rhomboides n. sp. is characterized by the presence of a rhomboid-like widening of the mid-ridge of lateral field at the level of vulva, a feature previously unknown within the genus. The population of the new species, composed only by females, has a rounded labial region with 4 to 5 annuli, robust stylet (31{\textendash}37μm long), short dorsal esophageal gland (9{\textendash}19μm) overlap of the intestine, vulva located slightly posterior to mid-body, and hemispherical or rounded tail shape with large phasmids located 3 to 5 annuli anterior to the level of anus. The hierarchical cluster analysis based on morphological features indicated that the new species closely resembles R. corsicus, R. gracilidens, and R. rugatocuticulatus. The DNA analyses of the D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA sequences of Rotylenchus rhomboides n. sp. show a close relationship with R. buxophilus, R. goodeyi, R. laurentinus, R. pumilus, and R. incultus, all of which can also be differentiated from the new species by morphological features. The combination of morphological, morphometric, and molecular characteristics confirmed the new species and the first report of R. buxophilus on yam (Dioscorea tokoro) in Belgium.}, keywords = {28S{\textendash}D2D3, coi, Dioscorea tokoro, Hierarchical cluster, its, morphology, Musa basjoo, phylogeny, Plant-parasitic, Rotylenchus, Systematic, taxonomy}, issn = {0022300X}, doi = {10.21307/jofnem-2019-023}, author = {Nguyen, Huu Tien and Trinh, Quang Phap and Couvreur, Marjolein and Singh, Phougeishangbam Rolish and Decraemer, Wilfrida and Bert, Wim} } @article {Wang2019, title = {Aphelenchoides medicagus n. sp. (Tylenchina: Aphelenchoididae) found in Medicago sativa imported into China from the USA}, journal = {Nematology}, volume = {21}, year = {2019}, pages = {709{\textendash}723}, abstract = {Aphelenchoides medicagus n. sp. isolated in Ningbo Port, P.R. China, from dried leaves and shoots of Medicago sativa imported from the USA, is described. It is characterised by a lateral field with four lines (three bands), stylet 9.0-12.0 μ m long, excretory pore situated at same level as, or slightly posterior, to the nerve ring, vagina sclerotised, female vulval flap absent, post-uterine sac short, spicules 9.2-14.2 μ m long (median curved line), rosethorn-shaped, apex and rostrum rounded, poorly developed, female and male tail conical with terminus bearing star-like processes. The new species belongs to the Group 3 category of Aphelenchoides species sensu Shahina. Phylogenetic analyses based on 18S, ITS and 28S D2-D3 of rDNA and mtCOI sequences confirmed its status as a new species and closely related to A. besseyi and A. fujianensis .}, keywords = {alfalfa, description, lucerne, molecular, morphology, Morphometrics, new species, phylogeny, plant-parasitic nematode, quarantine, SEM, taxonomy}, isbn = {1568541100003}, issn = {15685411}, doi = {10.1163/15685411-00003247}, author = {Wang, Zhen and Bert, Wim and Gu, Jianfeng and Couvreur, Marjolein and Li, Hongmei} }