@article {195,
	title = {Development and pharyngeal gland activities of Heterodera schachtii infecting Arabidopsis thaliana roots},
	journal = {NematologyNematology},
	volume = {4},
	year = {2002},
	note = {Tytgat, T De Meutter, J Vanholme, B Claeys, M Verreijdt, L Gheysen, G Coomans, APart 8},
	pages = {899-908},
	abstract = {As plant-nematode research continues to use the model host plant Arabidopsis thaliana more and more, we made a detailed life cycle study of Heterodera schachtii on this host, and investigated pharyngeal gland activities at the most crucial steps during the establishment of the parasitic interaction. In our experimental set up, in most cases induction of syncytia occurred during day 2, and the second moult during day 6. In pre-parasitic juveniles, both subventral glands contained numerous secretory granules, which gradually disappeared during intracellular migration of the juvenile through the roots. At the same time, both glands decreased in size and, after induction of the feeding cell, new kinds of secretory granules could be observed in the cytoplasm. During migration and induction of the feeding cell, the dorsal pharyngeal gland continuously increased in size. Secretory granules were already present in the dorsal gland of pre-parasitic juveniles, and a continuous synthesis of these granules was observed thereafter. Once the syncytium was induced, however, different types of secretory granules were formed.},
	isbn = {1388-5545},
	author = {Tytgat, T. and De Meutter, J. and Vanholme, B. and Claeys, M. and Verreijdt, L. and Gheysen, G. and Coomans, A.}
}
@article {211,
	title = {Sedentary endoparasitic nematodes as a model for other plant parasitic nematodes},
	journal = {NematologyNematology},
	volume = {2},
	year = {2000},
	note = {Tytgat, T De Meutter, J Gheysen, G Coomans, ASymposium on Biodiversity in th Phylum NematodaSep 17, 1999Ghent, belgiumUniv Gent, European Sci Fdn, Fund Sci Res FlandersPart 1},
	pages = {113-121},
	abstract = {Plant parasitic nematodes are known to cause a severe reduction in crop yield. Recently much effort is being put to engineering new nematode-resistant crop cultivars. Plant parasitic nematodes occur in three widely separated orders: Triplonchida, Dorylaimida and Tylenchida. All triplonchid and dorylaimid plant parasitic nematodes are migratory ectoparasites of roots. Within the Tylenchida, several different types of plant parasitism can be recognised. The sedentary endoparasites have the most complex interaction with their host, and are responsible for the vast majority of the agricultural damage. This causes most research to be concentrated on two groups of the sedentary endoparasitic nematodes: cyst- and root-knot nematodes. Both induce specialised feeding structures in the vascular cylinder of the plant root. The mechanism of phytoparasitism of the cyst- and root-knot nematodes is reviewed, of which some aspects will be applicable to the study of the other plant parasitic nematodes.},
	isbn = {1388-5545},
	author = {Tytgat, T. and De Meutter, J. and Gheysen, G. and Coomans, A.}
}
@article {170,
	title = {Secretions of plant-parasitic nematodes: a molecular update},
	journal = {GeneGene},
	volume = {332},
	year = {2004},
	note = {Vanholme, B De Meutter, J Tytgat, T Van Montagu, M Coomans, A Gheysen, G},
	month = {May},
	pages = {13-27},
	abstract = {The interaction between sedentary endoparasitic nematodes and plants is fascinating, because these animals have developed an ingenious way to manipulate the plant{\textquoteright}s gene regulation and metabolism to their own advantage. They are able to form highly specialized feeding structures in the plant root to satisfy their nutritional demands for development and reproduction. This ability makes them extremely successful parasites with severe consequences for agriculture. Triggered by these economical losses, detailed studies of the parasitic interaction have been performed, which resulted in an extensive descriptive knowledge. However, the underlying biochemical and molecular events of this intimate relationship have still not been elucidated. It is generally accepted that secretions produced by the nematode are responsible for the dramatic alteration of specific cells in the host plant. In the past few years, the identification of genes coding for secreted proteins was a breakthrough in plant nematode research. However, the available information is still too limited to allow the formulation of a comprehensive model, mainly because the sequences of many of these genes are novel with no similar sequence found in the existing databases. A new challenge in the coming years will be the functional analysis of these putative parasitism genes. (C) 2004 Elsevier B.V. All rights reserved.},
	isbn = {0378-1119},
	author = {Vanholme, B. and De Meutter, J. and Tytgat, T. and Van Montagu, M. and Coomans, A. and Gheysen, G.}
}
@article {173,
	title = {A new class of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in plant parasitic cyst nematodes},
	journal = {Molecular Plant-Microbe InteractionsMolecular Plant-Microbe Interactions},
	volume = {17},
	number = {8},
	year = {2004},
	note = {Tytgat, T Vanholme, B De Meutter, J Claeys, M Couvreur, M Vanhoutte, I Gheysen, G Van Criekinge, W Borgonie, G Coomans, A Gheysen, G},
	month = {Aug},
	pages = {846-852},
	abstract = {By performing cDNA AFLP on pre- and early parasitic juveniles, we identified genes encoding a novel type of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in the cyst nematode Heterodera schachtii. The proteins consist of three domains, a signal peptide for secretion, a mono-ubiquitin domain, and a short C-terminal positively charged domain. A gfp-fusion of this protein is targeted to the nucleolus in tobacco BY-2 cells. We hypothesize that the C-terminal peptide might have a regulatory function during syncytium formation in plant roots.},
	isbn = {0894-0282},
	author = {Tytgat, T. and Vanholme, B. and De Meutter, J. and Claeys, M. and Couvreur, M. and Vanhoutte, I. and Gheysen, G. and Van Criekinge, W. and Borgonie, G. and Coomans, A.}
}
@article {164,
	title = {An SXP/RAL-2 protein produced by the subventral pharyngeal glands in the plant parasitic root-knot nematode Meloidogyne incognita},
	journal = {Parasitology ResearchParasitology Research},
	volume = {95},
	number = {1},
	year = {2005},
	note = {Tytgat, T Vercauteren, I Vanholme, B De Meutter, J Vanhoutte, I Gheysen, G Borgonie, G Coomans, A Gheysen, G},
	month = {Jan},
	pages = {50-54},
	abstract = {Meloidogyne incognita is a major parasite of numerous plant families, including many crop species. Upon infection of the plant root, it induces several multinucleate giant cells by the injection of pharyngeal gland secretions into the root cells. In order to obtain a better understanding of the nematode-plant interaction, characterization of the pharyngeal gland secretions is a necessity. By differential display, a nematode gene was identified that encodes a new member of the SXP/RAL-2 protein family. The gene is specifically expressed in the subventral pharyngeal glands and the protein is most likely secreted.},
	isbn = {0932-0113},
	author = {Tytgat, T. and Vercauteren, I. and Vanholme, B. and De Meutter, J. and Vanhoutte, I. and Gheysen, G. and Borgonie, G. and Coomans, A.}
}
