@article {109,
	title = {Use of freeze-cracking in ontogenetic research in Macrostomum lignano (Macrostomida, Rhabditophora)},
	journal = {Development Genes and EvolutionDevelopment Genes and Evolution},
	volume = {219},
	number = {5},
	year = {2009},
	note = {Willems, Maxime Boone, Mieke Couvreur, Marjolein De Mulder, Katrien Van Ranst, Jelka Artois, Tom Borgonie, Gaetan},
	month = {May},
	pages = {273-279},
	abstract = {A method for studying whole mount flatworm embryos based on freeze-cracking of the eggs is described. This method allows successful immunohistological and immunocytological studies of whole mount embryos. It does not require the use of sharpened needles or a microinjection system to puncture the eggshell. Moreover, this method is more practical and less time-consuming than classical puncturing and much cheaper than the use of a microinjection system. The advantages of this method are illustrated by results of several immunolocalisation experiments in the macrostomid flatworm Macrostomum lignano. The optimal procedure and crucial steps for this method are discussed.},
	isbn = {0949-944X},
	author = {Willems, M. and Boone, M. and Couvreur, M. and De Mulder, K. and Van Ranst, J. and Artois, T. and Borgonie, G.}
}
@article {83,
	title = {First record of Nemertodermatida from Belgian marine waters},
	journal = {Belgian Journal of ZoologyBelgian Journal of Zoology},
	volume = {141},
	number = {1},
	year = {2011},
	pages = {50-54},
	author = {Boone, M. and Houthoofd, W. and Bert, W. and Artois, T.}
}
@article {84,
	title = {Spermatogenesis and the structure of the testes in Nemertodermatida},
	journal = {ZoomorphologyZoomorphology},
	volume = {130},
	number = {4},
	year = {2011},
	note = {Boone, Mieke Bert, Wim Claeys, Myriam Houthoofd, Wouter Artois, Tom},
	month = {Dec},
	pages = {273-282},
	abstract = {The ultrastructure of the testes in two representatives of the enigmatic taxon Nemertodermatida was studied using transmission electron microscopy. Nemertoderma westbladi has paired testes, which are delineated by lining cells. Within each testis, different follicles, each surrounded by a membrane-like structure, are found. Flagellophora apelti has genuinely follicular testes, consisting of several follicles, each containing a certain stage of spermatogenesis. As the gametes are not enclosed by a structure that can be called a true gonad, the structure of the testes differs from most bilaterian animals, but resembles the organization of gametogenic areas of ctenophores. Each stage of spermatogenesis in F. apelti is described, enabling the inference of the origin of the structures seen in mature spermatozoa. The overall structure of the mature spermatozoa is similar in all nemertodermatids and unique within the Metazoa: an elongated head containing the nucleus; a middle piece containing an axoneme, mitochondrial derivatives and in F. apelti granular derivatives; and a flagellar tail.},
	isbn = {0720-213X},
	author = {Boone, M. and Bert, W. and Claeys, M. and Houthoofd, W. and Artois, T.}
}
@article {86,
	title = {Distribution of proliferating cells and vasa-positive cells in the embryo of Macrostomum lignano (Rhabditophora, Platyhelminthes)},
	journal = {Belgian Journal of ZoologyBelgian Journal of Zoology},
	volume = {140},
	year = {2010},
	note = {Willems, Maxime Couvreur, Marjolein Boone, Mieke Houthoofd, Wouter Artois, Tom11th International Symposium on Flatworm BiologyJul 26-31, 2009Diepenbeek, BELGIUMS},
	month = {Jul},
	pages = {149-153},
	abstract = {The neoblast stem cell system of flatworms is considered to be unique within the animal kingdom. How this stem cell system arises during embryonic development is intriguing. Therefore we performed bromodeoxyuridine labelling on late stage embryos of Macrostomum lignano to assess when the pattern of proliferating cells within the embryo is comparable to that of hatchlings. This pattern can be found in late embryonic stages ( stage 8). We also used the freeze cracking method to perform macvasa embryonic labelling. Macvasa is a somatic and germ line stem cell marker. We showed macvasa protein distribution during the whole embryonic development. In the macvasa-positive blastomeres the protein is localized around the nucleus in the putative chromatoid bodies. However, at a specific embryonic stage, it is also ubiquitously present in the cytoplasm of some blastomeres. We compare our data with what is known from Schmidtea polychroa of the expression of the vasa-like gene SpolvlgA and the protein distribution of the chromatoid body component Spoltud-1. The embryonic origin of the somatic stem cell system and the germ line is discussed.},
	isbn = {0777-6276},
	author = {Willems, M. and Couvreur, M. and Boone, M. and Houthoofd, W. and Artois, T.}
}
@article {107,
	title = {Ontogeny of the Complex Sperm in the Macrostomid Flatworm Macrostomum lignano (Macrostomorpha, Rhabditophora)},
	journal = {Journal of MorphologyJournal of Morphology},
	volume = {270},
	number = {2},
	year = {2009},
	note = {Willems, Maxime Leroux, Frederic Claeys, Myriam Boone, Mieke Mouton, Stijn Artois, Tom Borgonie, Gaetan},
	month = {Feb},
	pages = {162-174},
	abstract = {Spermiogenesis in Macrostomum lignano (Macrostomorpha, Rhabditophora) is described using light- and electron microscopy of the successive stages in sperm development. Ovoid spermatids develop to highly complex, elongated sperm possessing an undulating distal (anterior) process (or "feeler"), bristles, and a proximal (posterior) brush. In particular, we present a detailed account of the morphology and ontogeny of the bristles, describing for the first time the formation of a highly specialized bristle complex consisting of several parts. This complex is ultimately reduced when sperm are mature. The implications of the development of this bristle complex on both sperm maturation and the evolution and function of the bristles are discussed. The assumed homology between bristles and flagellae questioned. J. Morphol. 270:162-174, 2009. (C) 2008 Wiley-Liss, Inc.},
	isbn = {0362-2525},
	author = {Willems, M. and Leroux, F. and Claeys, M. and Boone, M. and Mouton, S. and Artois, T. and Borgonie, G.}
}
