01501nas a2200193   4500008004100000020001400041245015100055210006900206260000800275300001200283490000800295520087100303100001701174700002101191700001701212700001601229700001601245856004601261       1994                        eng d  a0301-556400aTISSUE TREATMENT FOR WHOLE-MOUNT INTERNAL LECTIN STAINING IN THE NEMATODES CAENORHABDITIS-ELEGANS, PANAGROLAIMUS-SUPERBUS AND ACROBELOIDES-MAXIMUS0 aTISSUE TREATMENT FOR WHOLEMOUNT INTERNAL LECTIN STAINING IN THE   cJun  a379-3840 v1013 aFour different fixation schemes, using ten fluorescent-labelled lectins, were investigated for whole mount internal staining of three rhabditid nematodes: Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus. Acetone-only fixation was found to give strong and reproducible staining, which could be prevented either by periodate treatment of the organisms or by specific inhibitory sugars of the lectins under investigation. Whereas the use of either phosphate or TRIS buffers had no effect on the staining pattern or the fluorescence intensity, the incubation time as well as the in cubation temperature affected the staining reaction. The best results were obtained upon overnight incubation at 4 degrees C: the lectin staining could be inhibited in all cases, except for the intestinal brush border of C. elegans by the lectin of Lens culinaris.1 aBorgonie, G.1 aVanDriessche, E.1 aLink, C., D.1 aDeWaele, D.1 aCoomans, A.  uhttps://nematodes.myspecies.info/node/292